
Performance evaluation of a laboratory developed PCR test for quantitation of HIV-2 viral RNA.
Management of Human Immunodeficiency Virus Type 2 (HIV-2) infections present unique challenges due to low viral titers, slow disease progression, and poor response to standard antiviral therapies. The need for a nucleic acid assay to detect and quantify HIV-2 virus has led to the development of a number of molecular-based assays for detection and/or quantification of HIV-2 viral RNA in plasma in order to provide laboratory evidence of HIV-2 infection and viral loads for use in treatment decisions.
As HIV-2 is less pathogenic and transmissible than HIV-1 and has resistance to several of the antiretroviral drugs, delay of treatment is common. Cross sero-reactivity between HIV-1 and HIV-2 makes it difficult to distinguish between the two viruses based upon serological tests.
As such we developed a quantitative reverse transcription PCR (qRT-PCR) assay targeting the 5′ long terminal repeat of HIV-2 for detection and quantification of HIV-2 viral RNA in plasma to identify HIV-2 infection and for use in viral load monitoring. Serial dilutions of cultured HIV-2 virus demonstrated a wide dynamic range (10 to 100,000 copies/ml) with excellent reproducibility (standard deviation from 0.12-0.19), linearity (R2 = 0.9994), and a lower limit of detection at 79 copies/ml (NIH-Z). The assay is highly specific for HIV-2 Groups A and B and exhibits no cross reactivity to HIV-1, HBV or HCV.
Precision of the assay was demonstrated for the High (Mean = 6.41; SD = 0.12) and Medium (Mean = 4.46; SD = 0.13) HIV-2 positive controls. Replicate testing of clinical specimens showed good reproducibility above 1,000 copies/ml, with higher variability under 1,000 copies/ml. Analysis of 220 plasma samples from HIV-2 infected West African individuals demonstrated significantly lower viral loads than those observed in HIV-1 infections, consistent with results of previous studies. Slightly more than seven percent of clinical samples (7.3%) demonstrated viral loads above 100,000 copies/ml, while 37.3% of samples were undetectable.
The high sensitivity, specificity, precision, and linearity of the WRAIR qRT-PCR assay makes it well suited for detection and monitoring of HIV-2 RNA levels in plasma of infected individuals.

ichs2020
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C1434260 | Biochain | 40 reactions | EUR 376.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1734260 | Biochain | 40 reactions | EUR 376.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1534260 | Biochain | 40 reactions | EUR 540.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1534260-Cy | Biochain | 40 reactions | EUR 540.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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eNOS51-D-2 | Alpha Diagnostics | 2 ug | EUR 445.00 |
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02-715 | Sceti | 100ng | EUR 401.00 |
Description: The Mouse ; Testis is available in Europe and for worldwide shipping via Gentaur. |
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HTL-1381 | Alpha Diagnostics | 1 mg | EUR 773.00 |
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20-abx09801420ulSystems | Abbexa |
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M1164-100 | Biovision | EUR 294.00 |
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M1164-25 | Biovision | EUR 234.00 |
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M1165-100 | Biovision | EUR 354.00 |
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M1165-25 | Biovision | EUR 267.00 |
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M1168-100 | Biovision | EUR 381.00 |
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M1168-25 | Biovision | EUR 267.00 |
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M1169-100 | Biovision | EUR 441.00 |
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M1169-25 | Biovision | EUR 289.00 |
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HT05-090 | Alpha Diagnostics | 10 rxn | EUR 415.00 |
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HA-152 | Alpha Diagnostics | 10 rxn | EUR 415.00 |
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HA-218 | Alpha Diagnostics | 10 rxn | EUR 415.00 |
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C1634310 | Biochain | 40 reactions | EUR 621.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1634330 | Biochain | 40 reactions | EUR 621.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1634340 | Biochain | 40 reactions | EUR 621.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1634350 | Biochain | 40 reactions | EUR 621.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1634360 | Biochain | 40 reactions | EUR 621.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1634390 | Biochain | 40 reactions | EUR 621.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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7-06631 | CHI Scientific | 2µg | Ask for price |
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7-06632 | CHI Scientific | 10µg | Ask for price |
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7-06633 | CHI Scientific | 1mg | Ask for price |
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20-abx09801620ulSystems | Abbexa |
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20-abx09802120ulSystems | Abbexa |
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C1634370 | Biochain | 40 reactions | EUR 621.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1235004-10 | Biochain | 10 reactions | EUR 282.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1235010-10 | Biochain | 10 reactions | EUR 282.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1235023-10 | Biochain | 10 reactions | EUR 282.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1235035-10 | Biochain | 10 reactions | EUR 282.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1235086 | Biochain | 40 reactions | EUR 540.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1235090 | Biochain | 40 reactions | EUR 540.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1235142 | Biochain | 40 reactions | EUR 540.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1235149 | Biochain | 40 reactions | EUR 540.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1235152 | Biochain | 40 reactions | EUR 540.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1235161-10 | Biochain | 10 reactions | EUR 282.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1235164-10 | Biochain | 10 reactions | EUR 282.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1235178-10 | Biochain | 10 reactions | EUR 282.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1235183-10 | Biochain | 10 reactions | EUR 494.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1235188-10 | Biochain | 10 reactions | EUR 282.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1235189-10 | Biochain | 10 reactions | EUR 494.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1235201-10 | Biochain | 10 reactions | EUR 282.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1235206-10 | Biochain | 10 reactions | EUR 282.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1235218-10 | Biochain | 10 reactions | EUR 282.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1235218A-10 | Biochain | 10 reactions | EUR 282.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1235248 | Biochain | 40 reactions | EUR 540.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1235274 | Biochain | 40 reactions | EUR 540.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1236035Dia | Biochain | 40 reactions | EUR 668.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1236090Dia | Biochain | 40 reactions | EUR 668.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1236106Dia | Biochain | 40 reactions | EUR 668.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1236122Dia | Biochain | 40 reactions | EUR 668.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1236142Dia | Biochain | 40 reactions | EUR 668.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1236149Dia | Biochain | 40 reactions | EUR 668.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1236152Dia | Biochain | 40 reactions | EUR 668.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1236188Dia | Biochain | 40 reactions | EUR 668.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1236218Dia | Biochain | 40 reactions | EUR 668.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1236246Dia | Biochain | 40 reactions | EUR 668.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1236248Dia | Biochain | 40 reactions | EUR 668.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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AR0063 | BosterBio | 5mL | EUR 106.00 |
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C1236012Hd-4 | Biochain | 40 reactions | EUR 811.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1236013Hd-2 | Biochain | 40 reactions | EUR 668.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1236013Hd-4 | Biochain | 40 reactions | EUR 811.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1236020Hd-2 | Biochain | 40 reactions | EUR 668.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1236090Lup | Biochain | 40 reactions | EUR 668.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1236122Hd-2 | Biochain | 40 reactions | EUR 668.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1236122Lup | Biochain | 40 reactions | EUR 668.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1236142Hd-2 | Biochain | 40 reactions | EUR 668.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1236142Lup | Biochain | 40 reactions | EUR 668.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1236149Lup | Biochain | 40 reactions | EUR 668.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1236152Ld-1 | Biochain | 40 reactions | EUR 811.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1236152Ld-2 | Biochain | 40 reactions | EUR 811.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1236152Ld-3 | Biochain | 40 reactions | EUR 811.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1236152Ld-4 | Biochain | 40 reactions | EUR 811.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1236152Lup | Biochain | 40 reactions | EUR 668.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1236188Lup | Biochain | 40 reactions | EUR 668.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1236246Lup | Biochain | 40 reactions | EUR 668.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1236248Lup | Biochain | 40 reactions | EUR 668.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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BIO-65042 | Bioline | 30 Reactions | Ask for price |
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BIO-65043 | Bioline | 100 Reactions | Ask for price |
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BIO-65053 | Bioline | 50 Reactions | Ask for price |
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BIO-65053/S | Bioline | Sample | Ask for price |
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BIO-65054 | Bioline | 250 Reactions | Ask for price |
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G235 | ABM | 25 x 20 ul reactions | EUR 97.00 |
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G236 | ABM | 100 x 20 ul reactions | EUR 169.00 |
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G453 | ABM | 25 x 20 ul reactions | EUR 97.00 |
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G454 | ABM | 100 x 20 ul reactions | EUR 169.00 |
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G627 | ABM | 25 rxn (20 ul/rxn) | EUR 309.00 |
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M1167-100 | Biovision | EUR 952.00 |
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M1167-25 | Biovision | EUR 441.00 |
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PC34-310 | Alpha Diagnostics | 10 rxn | EUR 415.00 |
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2-96105 | CHI Scientific | 1 Kit | Ask for price |
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4-28105 | CHI Scientific | 1 Kit | Ask for price |
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1-CSB-EP006125HU | Cusabio |
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Description: Recombinant Human Cancer/testis antigen 1(CTAG1A) expressed in E.coli |
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1-CSB-EP006127HU | Cusabio |
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Description: Recombinant Human Cancer/testis antigen 2(CTAG2) expressed in E.coli |
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1-CSB-YP006125HU | Cusabio |
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Description: Recombinant Human Cancer/testis antigen 1(CTAG1A) expressed in Yeast |
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1-CSB-YP006127HUa4 | Cusabio |
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Description: Recombinant Human Cancer/testis antigen 2(CTAG2) expressed in Yeast |
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PROTQ9UGI8 | BosterBio | Regular: 10ug | EUR 317.00 |
Description: TES Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 441amino acids (1-421 a.a.) and having a molecular mass of 50.1 kDa. The TES protein is fused to 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques. |
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T1235260 | Biochain | 5 slides | EUR 338.00 |
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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T2235260 | Biochain | 5 slides | EUR 257.00 |
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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LYSATE0005 | BosterBio | 200ug | EUR 150.00 |
Description: This cell lysate is prepared from rat testis tissue using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C. |
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LYSATE0014 | BosterBio | 200ug | EUR 150.00 |
Description: This cell lysate is prepared from mouse testis tissue using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C. |
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X12009 | EpiGentek | 500 µg | Ask for price |
Description: kits suitable for this type of research |
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C1234003-10 | Biochain | 10 reactions | EUR 231.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1234004 | Biochain | 40 reactions | EUR 376.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1234010 | Biochain | 40 reactions | EUR 376.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1234035 | Biochain | 40 reactions | EUR 376.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1234086 | Biochain | 40 reactions | EUR 376.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1234090 | Biochain | 40 reactions | EUR 376.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1234106 | Biochain | 40 reactions | EUR 376.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1234122 | Biochain | 40 reactions | EUR 376.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1234142 | Biochain | 40 reactions | EUR 376.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1234149 | Biochain | 40 reactions | EUR 376.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1234152 | Biochain | 40 reactions | EUR 376.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1234160-10 | Biochain | 10 reactions | EUR 231.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1234183 | Biochain | 40 reactions | EUR 939.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1234188 | Biochain | 40 reactions | EUR 376.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1234200 | Biochain | 40 reactions | EUR 376.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1234201 | Biochain | 40 reactions | EUR 376.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1234206 | Biochain | 40 reactions | EUR 376.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1234218 | Biochain | 40 reactions | EUR 376.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1234246 | Biochain | 40 reactions | EUR 376.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1234248 | Biochain | 40 reactions | EUR 376.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1234261-10 | Biochain | 10 reactions | EUR 231.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1234263-10 | Biochain | 10 reactions | EUR 231.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1234264 | Biochain | 40 reactions | EUR 939.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1234265 | Biochain | 40 reactions | EUR 376.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1234267-10 | Biochain | 10 reactions | EUR 231.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1234268-10 | Biochain | 10 reactions | EUR 231.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1234274 | Biochain | 40 reactions | EUR 376.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1235171-10 | Biochain | 10 reactions | EUR 282.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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C1236003SubDia-10 | Biochain | 10 reactions | EUR 406.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
Use of real-time multiplex PCR, malaria rapid diagnostic test and microscopy to investigate the prevalence of Plasmodium species among febrile hospital patients in Sierra Leone.
- Malaria continues to affect over 200 million individuals every year, especially children in Africa. Rapid and sensitive detection and identification of Plasmodium parasites is crucial for treating patients and monitoring of control efforts.
- Compared to traditional diagnostic methods such as microscopy and rapid diagnostic tests (RDTs), DNA based methods, such as polymerase chain reaction (PCR) offer significantly higher sensitivity, definitive discrimination of Plasmodium species, and detection of mixed infections. While PCR is not currently optimized for routine diagnostics, its role in epidemiological studies is increasing as the world moves closer toward regional and eventually global malaria elimination.
- This study demonstrates the field use of a novel, ambient temperature-stabilized, multiplexed PCR assay in a small hospital setting in Sierra Leone.Blood samples from 534 febrile individuals reporting to a hospital in Bo, Sierra Leone, were tested using three methods: a commercial RDT, microscopy, and a Multiplex Malaria Sample Ready (MMSR) PCR designed to detect a universal malaria marker and species-specific markers for Plasmodium falciparum and Plasmodium vivax.
- A separate PCR assay was used to identify species of Plasmodium in samples in which MMSR detected malaria, but was unable to identify the species.MMSR detected the presence of any malaria marker in 50.2% of all tested samples with P. falciparum identified in 48.7% of the samples. Plasmodium vivax was not detected. Testing of MMSR P. falciparum-negative/universal malaria-positive specimens with a panel of species-specific PCRs revealed the presence of Plasmodium malariae (n = 2) and Plasmodium ovale (n = 2).
- The commercial RDT detected P. falciparum in 24.6% of all samples while microscopy was able to detect malaria in 12.8% of tested specimens.Wider application of PCR for detection of malaria parasites may help to fill gaps existing as a result of use of microscopy and RDTs.
- Due to its high sensitivity and specificity, species coverage, room temperature stability and relative low complexity, the MMSR assay may be useful for detection of malaria and epidemiological studies especially in low-resource settings.
![]() cDNA from Dog Normal Tissue: Testis |
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C1734260 | Biochain | 40 reactions | EUR 376.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
![]() cDNA from Human Adult Normal Tissue: Testis |
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C1234260 | Biochain | 40 reactions | EUR 376.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
![]() cDNA from Monkey (Rhesus) Normal Tissue: Testis |
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C1534260 | Biochain | 40 reactions | EUR 540.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
![]() cDNA from Monkey (Cynomolgus) Normal Tissue: Testis |
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C1534260-Cy | Biochain | 40 reactions | EUR 540.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
![]() Hyaluronidase (~300 U/mg: bovine testis) |
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RP-1469 | Alpha Diagnostics | 1500 IU | EUR 164.00 |
![]() Bovine Testis anion transporter 1, SLC26A8 ELISA KIT |
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ELI-15606b | Lifescience Market | 96 Tests | EUR 928.00 |
![]() Bovine Testis- specific gene 13 protein, TSGA13 ELISA KIT |
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ELI-16907b | Lifescience Market | 96 Tests | EUR 928.00 |
![]() Mouse ; Testis |
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02-715 | Sceti | 100ng | EUR 401.00 |
Description: The Mouse ; Testis is available in Europe and for worldwide shipping via Gentaur. |
![]() Bovine Testis- specific Y- encoded protein 1, TSPY1 ELISA KIT |
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ELI-51800b | Lifescience Market | 96 Tests | EUR 928.00 |
![]() cDNA Synthesis SuperMix |
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20-abx09801420ulSystems | Abbexa |
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![]() Evo? cDNA Kit |
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M1164-100 | Biovision | EUR 294.00 |
![]() Evo? cDNA Kit |
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M1164-25 | Biovision | EUR 234.00 |
![]() Novo? cDNA Kit |
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M1165-100 | Biovision | EUR 354.00 |
![]() Novo? cDNA Kit |
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M1165-25 | Biovision | EUR 267.00 |
![]() Evo? cDNA Supermix |
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M1168-100 | Biovision | EUR 381.00 |
![]() Evo? cDNA Supermix |
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M1168-25 | Biovision | EUR 267.00 |
![]() Novo? cDNA Supermix |
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M1169-100 | Biovision | EUR 441.00 |
![]() Novo? cDNA Supermix |
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M1169-25 | Biovision | EUR 289.00 |
![]() cDNA from Plant Normal Tissue: cDNA from Plant: Arabidopsis |
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C1634310 | Biochain | 40 reactions | EUR 621.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
![]() cDNA from Plant Normal Tissue: cDNA from Plant: Corn |
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C1634330 | Biochain | 40 reactions | EUR 621.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
![]() cDNA from Plant Normal Tissue: cDNA from Plant: Orange |
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C1634340 | Biochain | 40 reactions | EUR 621.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
![]() cDNA from Plant Normal Tissue: cDNA from Plant: Potato |
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C1634350 | Biochain | 40 reactions | EUR 621.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
![]() cDNA from Plant Normal Tissue: cDNA from Plant: Rice |
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C1634360 | Biochain | 40 reactions | EUR 621.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
![]() cDNA from Plant Normal Tissue: cDNA from Plant: Wheat |
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C1634390 | Biochain | 40 reactions | EUR 621.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
![]() First-Strand cDNA Synthesis SuperMix (cDNA up to 12 kb) |
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20-abx09801620ulSystems | Abbexa |
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![]() First-Strand cDNA Synthesis SuperMix (cDNA up to 15 kb) |
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20-abx09802120ulSystems | Abbexa |
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![]() cDNA from Plant Normal Tissue: cDNA from Plant: Soy bean |
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C1634370 | Biochain | 40 reactions | EUR 621.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
![]() cDNA Probe Diluent Solution |
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AR0063 | BosterBio | 5mL | EUR 106.00 |
![]() cDNA from Arteriosclerosis: Aorta |
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C1236012Hd-4 | Biochain | 40 reactions | EUR 811.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
![]() cDNA from hypertension: Artery |
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C1236013Hd-2 | Biochain | 40 reactions | EUR 668.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
![]() cDNA from Arteriosclerosis: Artery |
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C1236013Hd-4 | Biochain | 40 reactions | EUR 811.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
![]() cDNA from hypertension: Vein |
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C1236020Hd-2 | Biochain | 40 reactions | EUR 668.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
![]() cDNA from Lupus: Colon |
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C1236090Lup | Biochain | 40 reactions | EUR 668.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
![]() cDNA from hypertension: Heart |
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C1236122Hd-2 | Biochain | 40 reactions | EUR 668.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
![]() cDNA from Lupus: Heart |
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C1236122Lup | Biochain | 40 reactions | EUR 668.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
![]() cDNA from hypertension: Kidney |
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C1236142Hd-2 | Biochain | 40 reactions | EUR 668.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
![]() cDNA from Lupus: Kidney |
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C1236142Lup | Biochain | 40 reactions | EUR 668.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
![]() cDNA from Lupus: Liver |
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C1236149Lup | Biochain | 40 reactions | EUR 668.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
![]() cDNA from Asthma: Lung |
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C1236152Ld-1 | Biochain | 40 reactions | EUR 811.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
![]() cDNA from Bronchitis: Lung |
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C1236152Ld-2 | Biochain | 40 reactions | EUR 811.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
![]() cDNA from Emphysema: Lung |
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C1236152Ld-3 | Biochain | 40 reactions | EUR 811.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
![]() cDNA from Pneumonia: Lung |
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C1236152Ld-4 | Biochain | 40 reactions | EUR 811.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
![]() cDNA from Lupus: Lung |
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C1236152Lup | Biochain | 40 reactions | EUR 668.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
![]() cDNA from Lupus: Pancreas |
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C1236188Lup | Biochain | 40 reactions | EUR 668.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
![]() cDNA from Lupus: Spleen |
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C1236246Lup | Biochain | 40 reactions | EUR 668.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
![]() cDNA from Lupus: stomach |
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C1236248Lup | Biochain | 40 reactions | EUR 668.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
![]() Tetro cDNA Synthesis Kit |
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BIO-65042 | Bioline | 30 Reactions | Ask for price |
![]() Tetro cDNA Synthesis Kit |
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BIO-65043 | Bioline | 100 Reactions | Ask for price |
![]() SensiFAST cDNA Synthesis Kit |
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BIO-65053 | Bioline | 50 Reactions | Ask for price |
![]() SensiFAST cDNA Synthesis Kit |
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BIO-65053/S | Bioline | Sample | Ask for price |
![]() SensiFAST cDNA Synthesis Kit |
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BIO-65054 | Bioline | 250 Reactions | Ask for price |
![]() OneScriptPlus cDNA Synthesis Kit |
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G235 | ABM | 25 x 20 ul reactions | EUR 97.00 |
![]() OneScriptPlus cDNA Synthesis Kit |
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G236 | ABM | 100 x 20 ul reactions | EUR 169.00 |
![]() OneScriptPlus cDNA Synthesis SuperMix |
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G453 | ABM | 25 x 20 ul reactions | EUR 97.00 |
![]() OneScriptPlus cDNA Synthesis SuperMix |
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G454 | ABM | 100 x 20 ul reactions | EUR 169.00 |
![]() circRNA cDNA Synthesis Kit |
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G627 | ABM | 25 rxn (20 ul/rxn) | EUR 309.00 |
![]() Human eNOS cDNA probe |
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eNOS51-D-2 | Alpha Diagnostics | 2 ug | EUR 445.00 |
![]() Novo? Transcriptome cDNA Kit |
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M1167-100 | Biovision | EUR 952.00 |
![]() Novo? Transcriptome cDNA Kit |
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M1167-25 | Biovision | EUR 441.00 |
![]() Plant Tissue cDNA: Arabidopsis |
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PC34-310 | Alpha Diagnostics | 10 rxn | EUR 415.00 |
![]() Human Testis Tumor lysate |
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HTL-1381 | Alpha Diagnostics | 1 mg | EUR 773.00 |
![]() Rat Testis Tissue Lysate |
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LYSATE0005 | BosterBio | 200ug | EUR 150.00 |
Description: This cell lysate is prepared from rat testis tissue using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C. |
![]() Mouse Testis Tissue Lysate |
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LYSATE0014 | BosterBio | 200ug | EUR 150.00 |
Description: This cell lysate is prepared from mouse testis tissue using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C. |
![]() Mouse Testis Nuclear Extract |
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X12009 | EpiGentek | 500 µg | Ask for price |
Description: kits suitable for this type of research |
![]() Testis Tumor Tissue Array - 16 cases of testis tumors and 8 cases of non-tumor testis tissues. |
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Z7020096 | Biochain | 5 slides | EUR 1381.00 |
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
![]() cDNA Synthesis SuperMix for qPCR |
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20-abx09801920ulSystems | Abbexa |
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![]() cDNA from Alzheimer's Disease: Brain |
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C1236035Alz | Biochain | 40 reactions | EUR 668.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
![]() cDNA from Liver Cirrhosis: Brain |
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C1236035Lcs | Biochain | 40 reactions | EUR 668.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
![]() cDNA from Parkinson's Disease: Brain |
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C1236035Par | Biochain | 40 reactions | EUR 668.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
![]() cDNA from Dementia: Brain: Hippocampus |
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C1236052Dem | Biochain | 40 reactions | EUR 801.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
![]() cDNA from Depression: Brain: Hippocampus |
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C1236052Dep | Biochain | 40 reactions | EUR 801.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
![]() cDNA from Liver Cirrhosis: Colon |
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C1236090Lcs | Biochain | 40 reactions | EUR 668.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
![]() cDNA from Liver Cirrhosis: Esophagus |
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C1236106Lcs | Biochain | 40 reactions | EUR 668.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
![]() cDNA from Liver Cirrhosis: Heart |
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C1236122Lcs | Biochain | 40 reactions | EUR 668.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
![]() cDNA from hypertension: Interventricular Septum |
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C1236130Hd-2 | Biochain | 40 reactions | EUR 668.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
![]() cDNA from Liver Cirrhosis: Kidney |
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C1236142Lcs | Biochain | 40 reactions | EUR 668.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
![]() cDNA from Liver Cirrhosis: Liver |
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C1236149Lcs | Biochain | 40 reactions | EUR 668.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
![]() cDNA from Liver Cirrhosis: Lung |
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C1236152Lcs | Biochain | 40 reactions | EUR 668.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
![]() cDNA from Pulmonary Embolism: Lung |
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C1236152Ld-5 | Biochain | 40 reactions | EUR 811.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
![]() cDNA from Liver Cirrhosis: Diaphragm |
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C1236169Lcs | Biochain | 40 reactions | EUR 668.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
![]() cDNA from Liver Cirrhosis: Pancreas |
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C1236188Lcs | Biochain | 40 reactions | EUR 668.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
![]() cDNA from Liver Cirrhosis: Skin |
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C1236218Lcs | Biochain | 40 reactions | EUR 668.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
![]() cDNA from Lupus: Small Intestine |
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C1236226Lup | Biochain | 40 reactions | EUR 668.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
![]() cDNA from Lupus: Spinal Cord |
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C1236234Lup | Biochain | 40 reactions | EUR 668.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
![]() cDNA from Liver Cirrhosis: Spleen |
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C1236246Lcs | Biochain | 40 reactions | EUR 668.00 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
![]() Human Tumor Tissue: Breast cDNA |
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HT05-090 | Alpha Diagnostics | 10 rxn | EUR 415.00 |
![]() Human Adult cDNA Tissue: Lung |
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HA-152 | Alpha Diagnostics | 10 rxn | EUR 415.00 |
![]() Human Adult cDNA Tissue: Skin |
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HA-218 | Alpha Diagnostics | 10 rxn | EUR 415.00 |
![]() Total-Transcriptome cDNA Synthesis Kit |
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G904 | ABM | 25 reactions | EUR 224.00 |
![]() Total-Transcriptome cDNA Synthesis Kit |
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G905 | ABM | 100 reactions | EUR 544.00 |
![]() Mouse iNOS (macrophage) cDNA probe |
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iNOS61-D-2 | Alpha Diagnostics | 2 ug | EUR 445.00 |
![]() Evo? cDNA Kit (gDNA Removal) |
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M1166-100 | Biovision | EUR 381.00 |
![]() Monkey (Rhesus) cDNA Tissue: Thyroid |
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MR34-265 | Alpha Diagnostics | 10 rxn | EUR 415.00 |
![]() Accuris qMax cDNA Synthesis Kit |
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PR2100-C-100 | BenchMark | 1 PC | EUR 337.75 |
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![]() Accuris qMax cDNA Synthesis Kit |
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PR2100-C-25 | BenchMark | 1 PC | EUR 142.36 |
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![]() Accuris qMax cDNA Synthesis Kit |
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PR2100-C-250 | BenchMark | 1 PC | EUR 711.77 |
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![]() Accuris qMax cDNA Synthesis Kit |
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PR2100-C-S | BenchMark | 1 PC | EUR 77.76 |
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![]() amfiRivert cDNA Synthesis Master Mix |
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R5101-050 | GenDepot | 50 rxns | EUR 415.00 |
![]() amfiRivert cDNA Synthesis Master Mix |
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R5101-100 | GenDepot | 2X50 rxns | EUR 847.00 |
![]() amfiRivert cDNA Synthesis Master Mix |
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R5101-200 | GenDepot | 4X50 rxns | EUR 1211.00 |
![]() amfiRivet cDNA Synthesis 2X Buffer |
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R5102-050 | GenDepot | 500ul | EUR 134.00 |
![]() amfiRivet cDNA Synthesis 2X Buffer |
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R5102-100 | GenDepot | 2x500ul | EUR 192.00 |