Laboratory-Developed Real-Time RT-PCR test

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ichs2020

Comparison of SARS-CoV-2 Detection from Nasopharyngeal Swab Samples by the Roche cobas® 6800 SARS-CoV-2 Test and a Laboratory-Developed Real-Time RT-PCR test.

  • The urgent need to implement and rapidly expand testing for Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) infection has led to development of multiple assays. How these tests perform relative to one another is poorly understood. We evaluated the concordance between the Roche Diagnostics cobas® 6800 SARS-CoV-2 Test and a laboratory- developed Real-Time RT-PCR test (LDT) based on a modified Centers for Disease Control and Prevention (CDC) protocol, for the detection of SARS-CoV-2 in samples submitted to the Clinical Laboratories of the Mount Sinai Health System. 1006 Nasopharyngeal swabs in universal transport medium from persons under investigation were tested for SARS-CoV-2 as part of routine clinical care using the cobas SARS-CoV2 test with subsequent evaluation by the LDT.
  • Cycle threshold values were analyzed and interpreted as either positive (‘Detected’ or ‘Presumptive Positive’), negative (‘Not Detected’), inconclusive, or invalid. Statistical analysis was performed using GraphPad Prism 8. The cobas SARS-CoV2 test reported 706 positives and 300 negatives. The LDT reported 640 positives, 323 negatives, 34 inconclusive, and 9 invalids. When excluding inconclusive and invalid results, the overall percent agreement between the two platforms was 95.8%.
  • Cohen’s kappa coefficient (κ) was 0.904 (95 % CI 0.875-0.933), suggesting almost perfect agreement between both platforms. An overall discordance rate of 4.2% between the two systems may reflect differences in primer sequences, assay limit of detection, or other factors, highlighting the importance of comparing the performance of different testing platforms. This article is protected by copyright. All rights reserved.
ichs2020
ichs2020

Advance? PCR Mix
M1139-200 each
EUR 451.2

PCR Super Mix
abx299726-100g 100 µg Ask for price

PCR Super Mix
abx299726-20g 20 µg
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PCR Super Mix
abx299726-50g 50 µg Ask for price

Ready PCR Mix 2X
11170052-1 1 Unit (s)
EUR 56.92

Ready PCR Mix 2X
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Drop PCR Mix (Dye)
MBS278016-5mL 5mL
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Fire Start? PCR Mix
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Ready? PCR Mix-Dye
M1128-1000 each
EUR 614.4

Ready? PCR Mix-Dye
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EUR 229.2

Image Ready? PCR Mix
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Rigor? PCR Mix-Dye
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EUR 385.2

Whole Blood PCR Mix
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PowerPol 2X PCR Mix
RK20718 1mL
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Robust Ready? PCR Mix
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Breeze? PCR Mix-Dye
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Taq Mix (2x) (PCR Master Mix (2x))
50900-1 5 x1 ml
EUR 64.14
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Image Distant? PCR Mix
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Advance? PCR Mix-Dye
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2x PCR Red Mix
TBS4004 2.0 ml
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2x PCR Blue Mix
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PCR-EZ D-PCR MASTER MIX
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2X PCR Master Mix
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2X PCR Master Mix
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PCR Master Mix 2X
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PCR Master Mix 2X
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PCR Master Mix 2X
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HRM PCR Master Mix
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HRM PCR Master Mix
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MGI PCR Primer Mix
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EUR 6

PCR Master mix (SYBR)
abx460031-1096tests 10 × 96 tests Ask for price

PCR Master mix (SYBR)
abx460031-596tests 5 × 96 tests Ask for price

PCR Master mix (SYBR)
abx460031-96tests 96 tests
EUR 337.5

amfiSure PCR Master Mix
P0311-010 2x50 rxns
EUR 66

amfiSure PCR Master Mix
P0311-025 5X50 rxns
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amfiSure PCR Master Mix
P0311-050 10X50 rxns
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amfiSure PCR Master Mix
P0311-100 10x1ml
EUR 220

amfiSure PCR Master Mix
P0311-125 15X50 rxns
EUR 198

amfiSure PCR Master Mix
P0311-200 20x1ml
EUR 365

amfiSure PCR Master Mix
P0311-250 50x50 rxns
EUR 595

amfiSure PCR Master Mix
P0311-500 100x50 rxns
EUR 1010

PCR-Mix
L5051100 2.5 ml
EUR 52.5

amfiXpand PCR Master Mix
P0331-010 2X50 rxns
EUR 65

amfiXpand PCR Master Mix
P0331-025 5X50 rxns
EUR 118

amfiXpand PCR Master Mix
P0331-050 10X50 rxns
EUR 149

amfiXpand PCR Master Mix
P0331-250 50x50 rxns
EUR 430

Taq 2X PCR Mix V2
RK20607 5mL
EUR 3.82

Roseate PCR Master Mix (2x) (Taq Mix (2x))
46503 1 ml
EUR 23.09
Description: Part E

Roseate PCR Master Mix (2x) (Taq Mix (2x))
46503-1 5 x1 ml
EUR 70.13
Description: Part E

Roseate PCR Master Mix (2x) (Taq Mix (2x))
46503-2 5 x2.5 ml
EUR 145.39
Description: Part E

MyTaq Blood PCR Mix, 2x
BIO-25053/S Sample Ask for price

MyTaq Blood PCR Mix, 2x
BIO-25054 250 Reactions Ask for price

Taq 2x PCR Mix 100 rxn
BA01501 100rxn
EUR 112.8
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Fire Start? PCR Mix-Dye
M1142-200 each
EUR 451.2

HiScript-TS 2 × PCR Mix
RA103-01 40 rxns (25 μl/rxn)
EUR 87

HiScript-TS 2 × PCR Mix
RA103-02 200 rxns (25 μl/rxn)
EUR 344

HotTaq 2x PCR Mix 100 rxn
BA01503 100rxn
EUR 144
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

RedTaq 2x PCR Mix 100 rxn
BA01507 100rxn
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Description: High quality RedTaq polymerase for different PCR variations and downstream applications.

Robust Ready? PCR Mix-Dye
M1131-1000 each
EUR 738

Robust Ready? PCR Mix-Dye
M1131-200 each
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mRNAExpress Tail PCR mix (5uM)
MR-TAIL-PR 100 ul
EUR 111

2x Taq PCR Master Mix
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Description: Biotechnology

Taq PCR Master Mix (2x)
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Description: Ready-to-use Solution containing Taq DNA Polymerase, optimized reaction buffer, MgCl2 and dNTPs

Taq PCR Master Mix (2x)
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Description: Ready-to-use Solution containing Taq DNA Polymerase, optimized reaction buffer, MgCl2 and dNTPs

Taq PCR Master Mix (2x)
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Description: Ready-to-use Solution containing Taq DNA Polymerase, optimized reaction buffer, MgCl2 and dNTPs

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onTaq PCR Master Mix (2x)
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Description: Ready-to-use Solution containing onTaq DNA Polymerase, optimized reaction buffer, MgCl2 and dNTPs

onTaq PCR Master Mix (2x)
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Description: Ready-to-use Solution containing onTaq DNA Polymerase, optimized reaction buffer, MgCl2 and dNTPs

onTaq PCR Master Mix (2x)
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Description: Ready-to-use Solution containing onTaq DNA Polymerase, optimized reaction buffer, MgCl2 and dNTPs

tiTaq PCR Master Mix (2x)
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Description: Ready-to-use Solution containing tiTaq DNA Polymerase, optimized reaction buffer, MgCl2 and dNTPs

tiTaq PCR Master Mix (2x)
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Description: Ready-to-use Solution containing tiTaq DNA Polymerase, optimized reaction buffer, MgCl2 and dNTPs

tiTaq PCR Master Mix (2x)
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Description: Ready-to-use Solution containing tiTaq DNA Polymerase, optimized reaction buffer, MgCl2 and dNTPs

TaqMan 2X PCR Master Mix
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EcoTaq 2X PCR Master Mix
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EcoTaq 2X PCR Master Mix
ET-5 200 rxn
EUR 52.8
Description: EcoTaq 2x PCR Master Mix is designed for effective and fast amplification of templates, up to 10kb, with high fidelity. The mix is provided at 2x concentration and contains EcoTaq DNA Polymerase, dNTPs, and optimized buffer. The blue dye within the mix allows immediate loading of reaction mixture after PCR. The products are a mixture of blunt ends and single base (A) 3’ overhang and can be used for direct T/A cloning. Easy-to-use: Just add DNA template and primers to run your reaction. Load PCR products immediately after PCR without need for Loading Dyes. EcoTaq saves your time. Fast: PCR lasts less than an hour. EcoTaq saves your time. Ecological: Your PCR wastes less electricity since it lasts less an hour. EcoTaq helps protecting environment. Applications: High throughput PCR, Routine PCR with high reproducibility, T/A Cloning, RT-PCR

Hybrid PCR Master Mix (2x)
E2750-01 100reactions
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Description: Ready-to-use Solution containing Hybrid DNA Polymerase, optimized reaction buffer, MgCl2 and dNTPs

Hybrid PCR Master Mix (2x)
E2750-02 500reactions
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Hi-TEMP PCR MASTER MIX
MBT119-100R 1 unit
EUR 550.27
Description: Hi-TEMP PCR MASTER MIX

Hi-TEMP PCR MASTER MIX
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Description: Hi-TEMP PCR MASTER MIX

Hi-TEMP PCR MASTER MIX
MBT119-50R 1 unit
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Description: Hi-TEMP PCR MASTER MIX

OptiTaq PCR Master Mix (2x)
E2910-01 100reactions
EUR 42.51
Description: Ready-to-use Solution containing OptiTaq DNA Polymerase, optimized reaction buffer, MgCl2 and dNTPs

OptiTaq PCR Master Mix (2x)
E2910-02 200reactions
EUR 78.48
Description: Ready-to-use Solution containing OptiTaq DNA Polymerase, optimized reaction buffer, MgCl2 and dNTPs

The accuracy of the Montenegro skin test for leishmaniasis in PCR-negative patients.

As highly specific molecular biology-based techniques may not be sensitive enough for the diagnosis of American tegumentary leishmaniasis (ATL), clinicians frequently rely on immunological tests before treatment initiation. Hence, the correct combination of diagnostic tests is imperative for ATL diagnosis. We aimed to evaluate the accuracy of the Montenegro (Leishmanin) skin test (MST) in polymerase chain reaction (PCR)-negative patients to accurately detect ATL.Patients with a clinical picture compatible with ATL were divided into ATL (confirmed by lesion smear, culture indirect immunofluorescence, and/or histopathology) and no-ATL (diseases that can mimic leishmaniasis) groups. Conventional PCR for the minicircle kDNA of Leishmania was performed, and the MST was carried out for PCR-negative patients.Ninety-nine patients were included in this study, including 79 diagnosed with ATL (6 with mucocutaneous leishmaniasis) and 20 without ATL (no-ATL group).
The MST showed a high sensitivity of 90.0% (95% confidence interval [CI] = 69.90-97.21) in PCR-negative patients that was 10% higher than the sensitivity reported in PCR-positive population (79.66%; 95% CI = 67.73-87.96).One of the most important reasons for PCR negativity among patients with active ATL is the presence of a strong cellular immunological response, especially in chronic and mucocutaneous leishmaniasis. This reinforces the considerable utility of the tests that detect cellular responses against Leishmania antigens such as the MST in PCR-negative patients when the performance in screening situations is questionable.

Positive RT-PCRtests among discharged COVID-19 patients in Shenzhen, China.

Among all 209 discharged coronavirus 2019 patients in Shenzhen China between January 23 and February 21, 2020, there are 9 (4.3%) patients showed RT-PCR positive in throat swabs, 13 (6.2%) patients showed RT-PCR positive in anal swabs, and 22 (10.5%) positive in either type.
The time between discharge and positive RT-PCR tests is 4.7 days on average. These numbers shed light on the viral dynamics of COVID-19.

A case report of COVID-19 with false negative RT-PCR test: necessity of chest CT.

The definite diagnosis of corona virus disease 2019 (COVID-19) is based on the viral isolation or positive result of polymerase chain reaction (PCR) from sputum, or nasal swab, or throat swab. However, the sensitivity to detect COVID-19 of real time (RT)-PCR is reported to be lower than that of chest CT. We report a case of 34-year-old man who was diagnosed as negative for COVID-19 based on the four sequential RT-PCR tests of his pharyngeal swab.
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Monkeypox Virus Real Time PCR Kit
YJC70115NW-25T 25 tests/kit Ask for price
Description: The Bioperfectus Monkeypox Virus Real Time PCR Kit is an in vitro diagnostic test, based on real-time PCR technology, for the detection of DNA from the Monkeypox virus. Specimens can be obtained from human serum, lesion exudate samples and scab. BSL-2 facilities with standard BSL-2 work practices may be used for the test of t he Monkeypox virus.

Monkeypox Virus Real Time PCR Kit
ZD-0076-01 25 tests/kit Ask for price
Description: Monkeypox virus is the virus that causes the disease monkeypox in both humans and animals. Monkeypox virus is an Orthopoxvirus, a genus of the family Poxviridae that contains other viral species that target mammals. The virus is mainly found in tropical rainforest regions of central and West Africa. The primary route of infection is thought to be contact with the infected animals or their bodily fluids. The genome is not segmented and contains a single molecule of linear double-stranded DNA, 185000 nucleotides long. The Monkeypox Virus real time PCR Kit contains a specific ready-to-use system for the detection of the Monkeypox Virusthrough polymerase chain reaction (PCR) in the real-time PCR system. The master contains reagents and enzymes for the specific amplification of the Monkeypox Virus DNA. Fluorescence is emitted and measured by the real time systems ́ optical unit during the PCR. The detection of amplified Monkeypox Virus DNA fragment is performed in fluorimeter channel 530nm with the fluorescent quencher BHQ1. DNA extraction buffer is available in the kit and serum or lesion exudate samples are used for the extraction of the DNA. In addition, the kit contains a system to identify possible PCR inhibition by measuring the 560nm fluorescence of the internal control (IC). An external positive control defined as 1×10^7 copies/ml is supplied which allow the determination of the gene load.

Monkeypox Virus Real Time PCR Kit
ZD-0076-02 25 tests/kit Ask for price
Description: Monkeypox virus is the virus that causes the disease monkeypox in both humans and animals. Monkeypox virus is an Orthopoxvirus, a genus of the family Poxviridae that contains other viral species that target mammals. The virus is mainly found in tropical rainforest regions of central and West Africa. The primary route of infection is thought to be contact with the infected animals or their bodily fluids.The genome is not segmented and contains a single molecule of linear double-stranded DNA, 185000 nucleotides long.The Monkeypox Virus real time PCR Kit contains a specific ready-to-use system for the detection of the Monkeypox Virusthrough polymerase chain reaction (PCR) in the real-time PCR system. The master contains reagents and enzymes for the specific amplification of theMonkeypox VirusDNA. Fluorescence is emitted and measured by the real time systems ́ optical unit during the PCR. The detection of amplified Monkeypox Virus DNA fragment is performed in fluorimeter channelFAM with the fluorescent quencher BHQ1. DNA extraction buffer is available in the kit and serum or lesion exudate samples are used for the extraction of the DNA. In addition, the kit contains a system to identify possible PCR inhibition by measuring the HEX/VIC/JOE fluorescence of the internal control (IC). An external positive control defined as 1×107copies/ml is supplied which allow the determination of the gene load.

Staphilococcus aureus Real-TM Real Time PCR kit
B1772-96FRT 96
EUR 562.44

HBV Quantitative Real Time PCR Kit
GWB-LRB013 25 tests Ask for price

HBV Quantitative Real Time PCR Kit
GWB-LRB014 25 tests Ask for price

Toxoplasma Gondii Real Time PCR Kit
GWB-LRB049 25 tests Ask for price

Toxoplasma Gondii Real Time PCR Kit
GWB-LRB050 25 tests Ask for price

Human Lung Cancer PCR Primer Library
HLUCPL-I 1 set
EUR 657.6

Human Schwann Cell PCR Primer Library
HSCH-I 1 set
EUR 657.6

CHV Real Time PCR Detection kit
TRI-L09M1 32T
EUR 453.6

CHV Real Time PCR Detection kit
TRI-L09S1 16T
EUR 252

PCVⅡ Real Time PCR Detection kit
TRI-L29M1 48T
EUR 705.6

PCVⅡ Real Time PCR Detection kit
TRI-L29S1 24T
EUR 378

ASFV Real Time PCR Detection kit
TRI-L04M1C 48T
EUR 554.4

ASFV Real Time PCR Detection kit
TRI-L04S1C 24T
EUR 302.4

Babesia Real Time PCR Detection kit
TRI-L20M1 32T
EUR 453.6

Babesia Real Time PCR Detection kit
TRI-L20S1 16T
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Giardia Real Time PCR Detection kit
TRI-L26M1 32T
EUR 453.6

Giardia Real Time PCR Detection kit
TRI-L26S1 16T
EUR 252

Oncogenetics BRCA Panel Real-TM Real Time PCR kit
R-27-P-48FRT 48
EUR 1907.5

Brucella Real Time PCR Detection kit
TRI-L24M1 32T
EUR 453.6

Brucella Real Time PCR Detection kit
TRI-L24S1 16T
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Chlamydia Real Time PCR Detection kit
TRI-L15M1 32T
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Leptospira Real Time PCR Detection kit
TRI-L13M1 32T
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Leptospira Real Time PCR Detection kit
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Ehrilichia Real Time PCR Detection kit
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Edwardsiella Real Time PCR Detection kit
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EUR 1560.88

Chlamydia Pheumoniae (CP) Real Time PCR Kit
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Chlamydia Pheumoniae (CP) Real Time PCR Kit
GWB-LRB004 25 tests Ask for price

PRV (gE) Real Time PCR Detection kit
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PRV (gE) Real Time PCR Detection kit
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FHV-1 Real Time PCR Detection kit
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FHV-1 Real Time PCR Detection kit
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BP/MP/CP Multiplex Real Time PCR Kit
GWB-LRB007 25 tests Ask for price

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Vibrio Harveyi Real Time PCR Detection Kit
TRI-N04M1 32T
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Vibrio Harveyi Real Time PCR Detection Kit
TRI-N04S1 16T
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Babesia gibsoni Real Time PCR Detection kit
TRI-L21M1 32T
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Babesia gibsoni Real Time PCR Detection kit
TRI-L21S1 16T
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Bacteria/Vibrio Real Time PCR Detection Kit
TRI-N06M1 32T
EUR 529.2

Bacteria/Vibrio Real Time PCR Detection Kit
TRI-N06S1 16T
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Koi Herpesvirus Real Time PCR Detection kit
TRI-N18M1 32T
EUR 491.4

Koi Herpesvirus Real Time PCR Detection kit
TRI-N18S1 16T
EUR 289.8

HCV/HBV/HIV/HIV2 Real-TM Real Time PCR kit (lyophilized)
V50-50FRT-L 50
EUR 937.4

Toxoplasma gondii Real Time PCR Detection kit
TRI-L19M1 32T
EUR 453.6

Toxoplasma gondii Real Time PCR Detection kit
TRI-L19S1 16T
EUR 252

Baculovirus Penaei Real Time PCR Detection kit
TRI-N12M1 32T
EUR 491.4

Baculovirus Penaei Real Time PCR Detection kit
TRI-N12S1 16T
EUR 289.8

qTOWER3 auto Real Time PCR system
AJ844-00603-2 each
EUR 55045

HBV Quantitative & YMDD Mutation Real Time PCR Kit
GWB-LRB015 25 tests Ask for price

HBV Quantitative & YMDD Mutation Real Time PCR Kit
GWB-LRB016 25 tests Ask for price

HBV Quantitative & YMDD Mutation Real Time PCR Kit
GWB-LRB017 25 tests Ask for price

HBV Quantitative & YMDD Mutation Real Time PCR Kit
GWB-LRB018 25 tests Ask for price

Mycoplasma haemofelis Real Time PCR Detection kit
TRI-L17M1 32T
EUR 453.6

Mycoplasma haemofelis Real Time PCR Detection kit
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EUR 252

HBV Adefovir-resistant Mutants Real Time PCR Kit
GWB-LRB019 25 tests Ask for price

HBV Adefovir-resistant Mutants Real Time PCR Kit
GWB-LRB020 25 tests Ask for price

HBV Genetype B,C&D Real Time PCR Kit
GWB-LRB023 25 tests Ask for price

HBV Genetype B,C&D Real Time PCR Kit
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CPV and FPV Real Time PCR Detection kit
TRI-L18M1 32T
EUR 453.6

CPV and FPV Real Time PCR Detection kit
TRI-L18S1 16T
EUR 252

Transfusion-transmitted Virus (TTV) Real Time PCR Kit
GWB-LRB041 25 tests Ask for price

Transfusion-transmitted Virus (TTV) Real Time PCR Kit
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Bordetella bronchiseptica Real Time PCR Detection kit
TRI-L08M1 32T
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Bordetella bronchiseptica Real Time PCR Detection kit
TRI-L08S1 16T
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EMS/AHPND & EHP Real Time PCR Detection kit
TRI-N05M1 32T
EUR 529.2

EMS/AHPND & EHP Real Time PCR Detection kit
TRI-N05S1 16T
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FMD 2.1 Dry Real‐Time RT‐PCR
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EUR 800

Hepatopancreatic Parvovirus Real Time PCR Detection kit
TRI-N11M1 32T
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Hepatopancreatic Parvovirus Real Time PCR Detection kit
TRI-N11S1 16T
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Yellow head Virus Real Time PCR Detection Kit
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Yellow head Virus Real Time PCR Detection Kit
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HBV Precore Site 1896 Mutation Real Time PCR Kit
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HBV Precore Site 1896 Mutation Real Time PCR Kit
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Dengue Real-TM Real Time PCR kit for detection of Dengue Virus
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Taura Syndrome Virus Real Time PCR Detection Kit
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Taura Syndrome Virus Real Time PCR Detection Kit
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Feline leukemia virus Real Time PCR Detection kit
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Feline leukemia virus Real Time PCR Detection kit
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EUR 378

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