Laboratory-Developed Real-Time RT-PCR test

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ichs2020

Comparison of SARS-CoV-2 Detection from Nasopharyngeal Swab Samples by the Roche cobas® 6800 SARS-CoV-2 Test and a Laboratory-Developed Real-Time RT-PCR test.

  • The urgent need to implement and rapidly expand testing for Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) infection has led to development of multiple assays. How these tests perform relative to one another is poorly understood. We evaluated the concordance between the Roche Diagnostics cobas® 6800 SARS-CoV-2 Test and a laboratory- developed Real-Time RT-PCR test (LDT) based on a modified Centers for Disease Control and Prevention (CDC) protocol, for the detection of SARS-CoV-2 in samples submitted to the Clinical Laboratories of the Mount Sinai Health System. 1006 Nasopharyngeal swabs in universal transport medium from persons under investigation were tested for SARS-CoV-2 as part of routine clinical care using the cobas SARS-CoV2 test with subsequent evaluation by the LDT.
  • Cycle threshold values were analyzed and interpreted as either positive (‘Detected’ or ‘Presumptive Positive’), negative (‘Not Detected’), inconclusive, or invalid. Statistical analysis was performed using GraphPad Prism 8. The cobas SARS-CoV2 test reported 706 positives and 300 negatives. The LDT reported 640 positives, 323 negatives, 34 inconclusive, and 9 invalids. When excluding inconclusive and invalid results, the overall percent agreement between the two platforms was 95.8%.
  • Cohen’s kappa coefficient (κ) was 0.904 (95 % CI 0.875-0.933), suggesting almost perfect agreement between both platforms. An overall discordance rate of 4.2% between the two systems may reflect differences in primer sequences, assay limit of detection, or other factors, highlighting the importance of comparing the performance of different testing platforms. This article is protected by copyright. All rights reserved.
ichs2020
ichs2020

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Whole Blood PCR Mix
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Breeze? PCR Mix-Dye
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Distant? PCR Mix-Dye
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MyTaq Blood PCR Mix, 2x
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MyTaq Blood PCR Mix, 2x
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Fire Start? PCR Mix-Dye
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HiScript-TS 2 × PCR Mix
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HiScript-TS 2 × PCR Mix
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Robust Ready? PCR Mix-Dye
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Robust Ready? PCR Mix-Dye
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mRNAExpress Tail PCR mix (5uM)
MR-TAIL-PR 100 ul
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Taq 5x PCR Mix 12.5 mM
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amfiSure Prime PCR Master Mix
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amfiSure Prime PCR Master Mix
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amfiSure Prime PCR Master Mix
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amfiSure Prime PCR Master Mix
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amfiSure Prime PCR Master Mix
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amfiSure Advanced PCR Master Mix
P2311-025 5X50 rxns
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amfiSure Advanced PCR Master Mix
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amfiSure Advanced PCR Master Mix
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amfiSure Advanced PCR Master Mix
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amfiSure Advanced PCR Master Mix
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amfiSure ONE PCR Master Mix(2X)
P7000-005 5x1 ml
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amfiSure ONE PCR Master Mix(2X)
P7000-010 10x1 ml
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amfiSure ONE PCR Master Mix(2X)
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amfiSure ONE PCR Master Mix(2X)
P7000-100 100x1 ml
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Taq PCR Master Mix (2X, Red Dye)
BS9297 1ml
EUR 101.76

Taq PCR Master Mix (2X, Red Dye)
BS9298 5ml
EUR 227.04

Taq PCR Master Mix (2X, Blue Dye)
BS9295 1ml
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Taq PCR Master Mix (2X, Blue Dye)
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2×Taq PCR Master Mix(with dye)
K1034-1 1 ml
EUR 87.6

2×Taq PCR Master Mix(with dye)
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2×Taq PCR Master Mix(with dye)
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2×Taq PCR Master Mix(with dye)
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2×Taq PCR Master Mix(with dye)
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HotStart PCR Master mix (2X, Green Dye)
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HotStart PCR Master mix (2X, Green Dye)
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1-Drop PCR Mix (squeeze bottle, no pipet)
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amfiSure Ultra Fidelity PCR Master Mix(2X)
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amfiSure Ultra Fidelity PCR Master Mix(2X)
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Reverse Transcriptase RT PCR Master Mix (5X)
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Reverse Transcriptase RT PCR Master Mix (5X)
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amfiFusion High Fidelity PCR Master Mix(2X)
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Extra T7 gRNA PCR primer mix (5 uM)
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Taq 5x PCR Mix Ready-to-Load 12.5 mM
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2x Genotyping PCR Ready Master Mix (250 x 20µL rxn)
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Accuris High Fidelity PCR Master Mix, sample, 5 rxns
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2x GoldStar Best PCR Master Mix (with Dyes), 20 ul/rxn
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lyophilised real-time PCR Master Mix for dual labeled probes
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AzuraQuant cDNA Synthesis Kit - 25 Reactions
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Eco 48 Real Time PCR system including software and loading dock
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Eztime Real - time PCRPremix 2x,SYBR Green
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Eztime Real - time PCRPremix 2x,SYBRGreen , ROX
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Novel Coronavirus COVID-19 (2019-nCoV) Real Time RT-PCR Kit
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EUR 1189.2
Description: Novel Coronavirus (2019-nCoV) Real Time RT-PCR Kit is used for the qualitative detection of a novel coronavirus, which was identified in 2019 at Wuhan City, Hubei Province, China, in upper respiratory tract specimens (nasopharyngeal extracts, deep cough sputum, etc.) and lower respiratory tract specimens (alveoli irrigation fluid, etc.) by real time PCR systems.

Green Dye One Step qRT-PCR Master Mix For Quantitative Real Time PCR With ROX Dye
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EUR 392.4

TurboQ Real-time PCR Machine with Laptop
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EUR 33836.4

dsGreen for Real-Time PCR, 100×, 2 mL
61010 2 mL
EUR 435.6

dsGreen for Real-Time PCR, 100×, 5 mL
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dsGreen for Real-Time PCR, 100×, 10 mL
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dsGreen for Real-Time PCR, 100×, 1 mL
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Azura PureView 1 Kb DNA Ladder - 100 Lanes
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Azura PureView 1 Kb DNA Ladder - 500 Lanes
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Azura PureView 1 Kb DNA Ladder - 1000 Lanes
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Azura PureView 250bp DNA Ladder - 100 Lanes
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Azura PureView 250bp DNA Ladder - 500 Lanes
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Azura PureView 250bp DNA Ladder - 1000 Lanes
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Azura PureView 100bp DNA Ladder - 100 Lanes
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Azura PureView 100bp DNA Ladder - 500 Lanes
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Azura PureView 100bp DNA Ladder - 1000 Lanes
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Azura PureView 50bp DNA Ladder - 100 Lanes
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EUR 140.4

Azura PureView 50bp DNA Ladder - 500 Lanes
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Azura PureView 50bp DNA Ladder - 1000 Lanes
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AzuraView GreenFast qPCR Blue Mix Lo Rox - 200 Rxn
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EUR 214.8

AzuraView GreenFast qPCR Blue Mix Lo Rox - 500 Rxn
AZ-2305 500 Rxn
EUR 384

AzuraView GreenFast qPCR Blue Mix Lo Rox - 2000 Rxn
AZ-2320 2000 Rxn
EUR 1195.2

AzuraView GreenFast qPCR Blue Mix Lo Rox - 5000 Rxn
AZ-2325 5000 Rxn
EUR 2817.6

AzuraView GreenFast qPCR Blue Mix Hi Rox - 200 Rxn
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EUR 214.8

AzuraView GreenFast qPCR Blue Mix Hi Rox - 500 Rxn
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AzuraView GreenFast qPCR Blue Mix Hi Rox - 2000 Rxn
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AzuraView GreenFast qPCR Blue Mix Hi Rox - 5000 Rxn
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Mouse Oxidative Stress Primer Library
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Mouse T-Cell Receptor Signaling Primer Library
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Mouse TGF Beta Signaling Primer Library
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Mouse Tumor Invasion/Metastasis Primer Library
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Mouse Tumor Immunity Primer Library
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Mouse p53 Signaling Primer Library
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Mouse WNT Signaling Primer Library
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Mouse Apoptosis Primer Library
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Mouse Ras Signaling Primer Library
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Mouse Oncogene Primer Library
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Mouse Integrin Signaling Primer Library
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Mouse Jak/Stat Signaling Primer Library
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Mouse MAP Kinase Signaling Primer Library
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Mouse Myeloid Derived Suppressor Cell Primer Library
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Mouse NFKappaB Primer Library
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Mouse Notch Signaling Primer Library
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Mouse Neurotransmitter Signaling Primer Library
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Human T-Cell Anergy/Immune Tolerance Primer Library
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Human T-Cell Receptor Signaling Primer Library
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Human Th1 and Th2 Cell Differentiation Primer Library
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Human Tumor Invasion/Metastasis Primer Library
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Human Tumor Immunity Primer Library
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Human Toll-like Receptor Signaling Primer Library
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Human TNF Signaling Primer Library
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Human p53 Signaling Primer Library
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Human WNT Signaling Primer Library
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Human Cytoskeleton Primer Library
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Human Stem Cell Primer Library
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Human SMAD Signaling Primer Library
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Human Statin Signaling Library
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Human Signal Transduction Primer Library
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Human DNA Repair Primer Library I
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Human DNA Repair Primer Library II
HDRL-II 1 set
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Human Drug Transporter I Primer Library
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Human Drug Detoxication II
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Human PI3K-AKT Signaling Primer Library
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Human Amino Acid Metabolism Primer Library
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Human Androgen Signaling Primer Library
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Human Angiogenesis Primer Library
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Human Antigen Processing and Presentation Primer Library
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Human Atherosclerosis Primer Library
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Human Adherin - Tight Junction Primer Library
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Human Autophagy Primer Library
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Human Alzheimer's Disease Primer Library
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Human B-Cell Receptor Signaling Primer Library
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