
Comparison of SARS-CoV-2 Detection from Nasopharyngeal Swab Samples by the Roche cobas® 6800 SARS-CoV-2 Test and a Laboratory-Developed Real-Time RT-PCR test.
- The urgent need to implement and rapidly expand testing for Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) infection has led to development of multiple assays. How these tests perform relative to one another is poorly understood. We evaluated the concordance between the Roche Diagnostics cobas® 6800 SARS-CoV-2 Test and a laboratory- developed Real-Time RT-PCR test (LDT) based on a modified Centers for Disease Control and Prevention (CDC) protocol, for the detection of SARS-CoV-2 in samples submitted to the Clinical Laboratories of the Mount Sinai Health System. 1006 Nasopharyngeal swabs in universal transport medium from persons under investigation were tested for SARS-CoV-2 as part of routine clinical care using the cobas SARS-CoV2 test with subsequent evaluation by the LDT.
- Cycle threshold values were analyzed and interpreted as either positive (‘Detected’ or ‘Presumptive Positive’), negative (‘Not Detected’), inconclusive, or invalid. Statistical analysis was performed using GraphPad Prism 8. The cobas SARS-CoV2 test reported 706 positives and 300 negatives. The LDT reported 640 positives, 323 negatives, 34 inconclusive, and 9 invalids. When excluding inconclusive and invalid results, the overall percent agreement between the two platforms was 95.8%.
- Cohen’s kappa coefficient (κ) was 0.904 (95 % CI 0.875-0.933), suggesting almost perfect agreement between both platforms. An overall discordance rate of 4.2% between the two systems may reflect differences in primer sequences, assay limit of detection, or other factors, highlighting the importance of comparing the performance of different testing platforms. This article is protected by copyright. All rights reserved.

ichs2020
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M1134-200 | Biovision | EUR 267.00 |
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M1136-200 | Biovision | EUR 294.00 |
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M1139-200 | Biovision | EUR 376.00 |
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BS294 | Bio Basic | 100RXN, 100prep | EUR 93.50 |
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M1128-1000 | Biovision | EUR 512.00 |
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M1128-200 | Biovision | EUR 191.00 |
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M1129-200 | Biovision | EUR 294.00 |
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M1130-1000 | Biovision | EUR 615.00 |
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M1130-200 | Biovision | EUR 213.00 |
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M1133-200 | Biovision | EUR 321.00 |
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M1135-200 | Biovision | EUR 267.00 |
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M1137-200 | Biovision | EUR 294.00 |
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M1141-200 | Biovision | EUR 376.00 |
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M1143-200 | Biovision | EUR 321.00 |
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P0311-010 | GenDepot | 2x50 rxns | EUR 126.00 |
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P0311-025 | GenDepot | 5X50 rxns | EUR 165.00 |
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P0311-050 | GenDepot | 10X50 rxns | EUR 219.00 |
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P0311-100 | GenDepot | 10x1ml | EUR 286.00 |
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P0311-125 | GenDepot | 15X50 rxns | EUR 278.00 |
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P0311-200 | GenDepot | 20x1ml | EUR 469.00 |
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P0311-250 | GenDepot | 50x50 rxns | EUR 752.00 |
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P0311-500 | GenDepot | 100x50 rxns | EUR 1258.00 |
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P0331-010 | GenDepot | 2X50 rxns | EUR 151.00 |
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P0331-025 | GenDepot | 5X50 rxns | EUR 273.00 |
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P0331-050 | GenDepot | 10X50 rxns | EUR 448.00 |
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BIO-25053/S | Bioline | Sample | Ask for price |
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BIO-25054 | Bioline | 250 Reactions | Ask for price |
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MR-TAIL-PR | SBI | 100 ul | EUR 164.00 |
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P1311-025 | GenDepot | 5X50 rxns | EUR 161.00 |
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P1311-050 | GenDepot | 10X50 rxns | EUR 219.00 |
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P1311-125 | GenDepot | 15X50 rxns | EUR 273.00 |
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P1311-250 | GenDepot | 50x50 rxns | EUR 739.00 |
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P1311-500 | GenDepot | 100x50 rxns | EUR 1238.00 |
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M1131-1000 | Biovision | EUR 615.00 |
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P2311-025 | GenDepot | 5X50 rxns | EUR 165.00 |
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P2311-050 | GenDepot | 10X50 rxns | EUR 223.00 |
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P2311-125 | GenDepot | 15X50 rxns | EUR 282.00 |
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P2311-250 | GenDepot | 50x50 rxns | EUR 752.00 |
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P2311-500 | GenDepot | 100x50 rxns | EUR 1279.00 |
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BA01501 | Bioatlas | 100rxn | EUR 94.00 |
Description: High quality Taq polymerase for different PCR variations and downstream applications. |
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BA01503 | Bioatlas | 100rxn | EUR 120.00 |
Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
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BA01507 | Bioatlas | 100rxn | EUR 146.00 |
Description: High quality RedTaq polymerase for different PCR variations and downstream applications. |
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Description: High quality Taq polymerase for different PCR variations and downstream applications. |
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P7000-005 | GenDepot | 5x1 ml | EUR 219.00 |
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P7000-050 | GenDepot | 50x1 ml | EUR 1211.00 |
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PCR-MPX218-48D | Bioingentech | 50T | EUR 425.80 |
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Description: An conventional PCR kit for detection of MULTIPLEX KIT PCR MASTITIS |
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PCR-MPX218-96D | Bioingentech | 100T | EUR 521.50 |
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Description: An conventional PCR kit for detection of MULTIPLEX KIT PCR MASTITIS |
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PCR-MPX401-48D | Bioingentech | 50T | EUR 425.80 |
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Description: An conventional PCR kit for detection of MULTIPLEX KIT PCR Babesia & Theileria |
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PCR-MPX401-96D | Bioingentech | 100T | EUR 521.50 |
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Description: An conventional PCR kit for detection of MULTIPLEX KIT PCR Babesia & Theileria |
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CAS510A-PR | SBI | 50 assays | EUR 153.00 |
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Q5601-010 | GenDepot | 10x1ml | EUR 442.00 |
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Q5601-050 | GenDepot | 50x1ml | EUR 1670.00 |
The accuracy of the Montenegro skin test for leishmaniasis in PCR-negative patients.
As highly specific molecular biology-based techniques may not be sensitive enough for the diagnosis of American tegumentary leishmaniasis (ATL), clinicians frequently rely on immunological tests before treatment initiation. Hence, the correct combination of diagnostic tests is imperative for ATL diagnosis. We aimed to evaluate the accuracy of the Montenegro (Leishmanin) skin test (MST) in polymerase chain reaction (PCR)-negative patients to accurately detect ATL.Patients with a clinical picture compatible with ATL were divided into ATL (confirmed by lesion smear, culture indirect immunofluorescence, and/or histopathology) and no-ATL (diseases that can mimic leishmaniasis) groups. Conventional PCR for the minicircle kDNA of Leishmania was performed, and the MST was carried out for PCR-negative patients.Ninety-nine patients were included in this study, including 79 diagnosed with ATL (6 with mucocutaneous leishmaniasis) and 20 without ATL (no-ATL group).
The MST showed a high sensitivity of 90.0% (95% confidence interval [CI] = 69.90-97.21) in PCR-negative patients that was 10% higher than the sensitivity reported in PCR-positive population (79.66%; 95% CI = 67.73-87.96).One of the most important reasons for PCR negativity among patients with active ATL is the presence of a strong cellular immunological response, especially in chronic and mucocutaneous leishmaniasis. This reinforces the considerable utility of the tests that detect cellular responses against Leishmania antigens such as the MST in PCR-negative patients when the performance in screening situations is questionable.
Positive RT-PCRtests among discharged COVID-19 patients in Shenzhen, China.
Among all 209 discharged coronavirus 2019 patients in Shenzhen China between January 23 and February 21, 2020, there are 9 (4.3%) patients showed RT-PCR positive in throat swabs, 13 (6.2%) patients showed RT-PCR positive in anal swabs, and 22 (10.5%) positive in either type.
The time between discharge and positive RT-PCR tests is 4.7 days on average. These numbers shed light on the viral dynamics of COVID-19.
A case report of COVID-19 with false negative RT-PCR test: necessity of chest CT.
The definite diagnosis of corona virus disease 2019 (COVID-19) is based on the viral isolation or positive result of polymerase chain reaction (PCR) from sputum, or nasal swab, or throat swab. However, the sensitivity to detect COVID-19 of real time (RT)-PCR is reported to be lower than that of chest CT. We report a case of 34-year-old man who was diagnosed as negative for COVID-19 based on the four sequential RT-PCR tests of his pharyngeal swab.
Chest CT showed patchy ground-glass opacity on admission, and it rapidly progressed to segmental mixed consolidation and ground-glass opacity 3 days after admission, and it resolved in left upper lobe, but showed multifocal ground-glass opacities 7 days after admission, and they resolved within 2 weeks. The fifth RT-PCR test finally revealed positive results at the fifth day after admission.
It is difficult to distinguish COVID-19 pneumonia from other viral pneumonia on CT findings alone; however, we emphasize the utility of chest CT to detect early change of COVID-19 in cases which RT-PCR tests show negative results
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81010 | lumiprobe | 10 mL | EUR 869.00 |
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71010 | lumiprobe | 5 mL | EUR 625.00 |
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51010 | lumiprobe | 1.5 mL | EUR 331.00 |
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41010 | lumiprobe | 1 mL | EUR 249.00 |
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11010 | lumiprobe | 100 uL | EUR 59.00 |
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61010 | lumiprobe | 2 mL | EUR 363.00 |
![]() PMA Real-Time PCR Bacterial Viability Kit - Salmonella enterica (invA) |
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31033 | Biotium | 1kit | EUR 428.00 |
Description: Minimum order quantity: 1 unit of 1kit |
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31034 | Biotium | 1kit | EUR 399.00 |
Description: Minimum order quantity: 1 unit of 1kit |
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31035 | Biotium | 1kit | EUR 399.00 |
Description: Minimum order quantity: 1 unit of 1kit |
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31036 | Biotium | 1kit | EUR 399.00 |
Description: Minimum order quantity: 1 unit of 1kit |
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31050 | Biotium | 1kit | EUR 428.00 |
Description: Minimum order quantity: 1 unit of 1kit |
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31051 | Biotium | 1kit | EUR 399.00 |
Description: Minimum order quantity: 1 unit of 1kit |
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31053 | Biotium | 1kit | EUR 428.00 |
Description: Minimum order quantity: 1 unit of 1kit |
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31033-X | Biotium | 1kit | EUR 428.00 |
Description: Minimum order quantity: 1 unit of 1kit |
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31050-X | Biotium | 1kit | EUR 428.00 |
Description: Minimum order quantity: 1 unit of 1kit |
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31051-X | Biotium | 1kit | EUR 428.00 |
Description: Minimum order quantity: 1 unit of 1kit |
![]() Novel Coronavirus COVID-19 (2019-nCoV) Real Time RT-PCR Kit |
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RR-0478-02 | Liferiver | 25 tests/kit | EUR 991.00 |
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Description: Novel Coronavirus (2019-nCoV) Real Time RT-PCR Kit is used for the qualitative detection of a novel coronavirus, which was identified in 2019 at Wuhan City, Hubei Province, China, in upper respiratory tract specimens (nasopharyngeal extracts, deep cough sputum, etc.) and lower respiratory tract specimens (alveoli irrigation fluid, etc.) by real time PCR systems. |
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20-abx097601 | Abbexa |
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![]() PMA Real-Time PCR Bacterial Viability Kit - E. coli O157:H7 (Z3276) |
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31037 | Biotium | 1kit | EUR 428.00 |
Description: Minimum order quantity: 1 unit of 1kit |
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DD2H | Detroit R&D | 1 Kit | EUR 430.00 |
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DD2M | Detroit R&D | 1 Kit | EUR 430.00 |
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DD2R | Detroit R&D | 1 Kit | EUR 430.00 |
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M034-Kit | TOKU-E | Kit | EUR 266.00 |
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HLUCPL-I | Real Time Primers | 1 set | EUR 548.00 |
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HSCH-I | Real Time Primers | 1 set | EUR 548.00 |
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A26979 | GeneOn | 1x100 pcs | EUR 186.00 |
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FYT105-100P | Yeastern Biotech | 100 Preps 1.25 ml | Ask for price |
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31037-X | Biotium | 1kit | EUR 428.00 |
Description: Minimum order quantity: 1 unit of 1kit |
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FYT103-100P | Yeastern Biotech | 100 Preps 1.25 ml | Ask for price |
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FYT103-400P | Yeastern Biotech | 400 Preps 1.25 ml x 4 | Ask for price |
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FYT104-100P | Yeastern Biotech | 100 Preps 1.25 ml | Ask for price |
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FYT104-400P | Yeastern Biotech | 400 Preps 1.25 ml x 4 | Ask for price |
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FYT105-400P | Yeastern Biotech | 400 Preps 1.25 ml x 4 | Ask for price |
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FYT106-100P | Yeastern Biotech | 100 Preps 1.25 ml | Ask for price |
![]() 0,1ML THIN WALL TUBES 8 PER STRIP. CLEAR & REAL TIME STRIP CAPS |
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PCR-0108-LP-RT-C | CORNING | 125/pk | EUR 709.00 |
Description: PCR Plates & Tubes; PCR Strip Tubes and Strip Caps - Axygen |
![]() 0,1ML THIN WALL TUBES 8 PER STRIP. WHITE & REAL TIME STRIP CAPS |
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PCR-0108-LP-RT-W | CORNING | 125/pk | EUR 770.00 |
Description: PCR Plates & Tubes; PCR Strip Tubes and Strip Caps - Axygen |
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MB1004-100Reactions | BosterBio | 100 Reactions | EUR 327.00 |
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20-abx097657 | Abbexa |
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![]() hsa-mir-34b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097659 | Abbexa |
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![]() hsa-mir-92a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097661 | Abbexa |
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![]() hsa-mir-92b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097662 | Abbexa |
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![]() hsa-mir-93 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097663 | Abbexa |
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![]() hsa-mir-95 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097664 | Abbexa |
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![]() hsa-mir-96 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097665 | Abbexa |
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![]() hsa-mir-98 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097666 | Abbexa |
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![]() hsa-mir-99a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097667 | Abbexa |
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![]() hsa-mir-99b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097669 | Abbexa |
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![]() hsa-mir-100 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097671 | Abbexa |
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![]() hsa-mir-101 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097673 | Abbexa |
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![]() hsa-mir-103 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097675 | Abbexa |
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![]() hsa-mir-105 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097676 | Abbexa |
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![]() hsa-mir-106a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097677 | Abbexa |
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![]() hsa-mir-106b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097678 | Abbexa |
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![]() hsa-mir-107 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097679 | Abbexa |
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![]() hsa-mir-124 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097680 | Abbexa |
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![]() hsa-mir-125a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097681 | Abbexa |
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![]() hsa-mir-127 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097686 | Abbexa |
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![]() hsa-mir-129 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097689 | Abbexa |
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![]() hsa-mir-130a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097690 | Abbexa |
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![]() hsa-mir-130b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097691 | Abbexa |
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![]() hsa-mir-132 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097692 | Abbexa |
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![]() hsa-mir-133a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097693 | Abbexa |
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![]() hsa-mir-133b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097694 | Abbexa |
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![]() hsa-mir-134 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097695 | Abbexa |
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![]() hsa-mir-135b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097696 | Abbexa |
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![]() hsa-mir-137 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097699 | Abbexa |
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![]() hsa-mir-138 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097700 | Abbexa |
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![]() hsa-mir-139 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097701 | Abbexa |
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![]() hsa-mir-140 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097702 | Abbexa |
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![]() hsa-mir-141 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097703 | Abbexa |
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![]() hsa-mir-143 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097706 | Abbexa |
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![]() hsa-mir-145 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097708 | Abbexa |
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![]() hsa-mir-146a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097709 | Abbexa |
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![]() hsa-mir-149 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097711 | Abbexa |
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![]() hsa-mir-150 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097712 | Abbexa |
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![]() hsa-mir-151 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097714 | Abbexa |
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![]() hsa-mir-152 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097715 | Abbexa |
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![]() hsa-mir-153 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097716 | Abbexa |
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![]() hsa-mir-154 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097717 | Abbexa |
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![]() hsa-mir-181a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097719 | Abbexa |
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![]() hsa-mir-181b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097720 | Abbexa |
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![]() hsa-mir-181c Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097721 | Abbexa |
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![]() hsa-mir-181d Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097722 | Abbexa |
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![]() hsa-mir-182 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097723 | Abbexa |
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![]() hsa-mir-183 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097724 | Abbexa |
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![]() hsa-mir-184 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097725 | Abbexa |
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![]() hsa-mir-185 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097726 | Abbexa |
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