The clinical characteristic of eight patients of COVID-19 with positive RT-PCR test after discharge.
- Corona virus disease 2019 (COVID-19) was caused by Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2). The phenomenon of positive real time reverse transcription polymerase chain reaction (RT-PCR) result of SARS-CoV-2 in recovered patients had occurred and the research about these patients was rare.
- In our study, we did a retrospective review of medical records from COVID-19 patients admitted to one ward of Tongji Hospital of Hua Zhong University of Science and Technology from 10 February to 13 April 2020. From 10 February to 13 April 2020, there were 108 patients of COVID-19 admitted in the one ward of Tongji Hospital. Among them, eight cases were readmission patients because the RT-PCR result of SARS-CoV-2 was positive again after discharge. On the second admission, they had no symptoms and their chest CT was almost normal.
- Data from laboratory tests of the re-admission patients showed that all eight patients had normal white blood cell count, lymphocyte count. The inflammatory factors like procalcitonin and interleukin 6 were normal. After treatment, two patients met the standard and were discharged. The other six patients were still in the hospital because their RT-PCR of SARS-CoV-2 did not get three consecutive negative results and the course of two patients had persisted more than 90 days.
- We still needed to be alert that these patients could infect other people as a source of infection, and we also needed to be alert that these patients become chronic virus carriers. It also aroused our concern about the discharge standard of COVID-19. This article is protected by copyright. All rights reserved.
ichs2020
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
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Fast Pro QPCR SuperMix Kit - ROX premixed |
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
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T-Pro Genomic DNA Midi Kit (20) |
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T-Pro Genomic DNA Mini Kit (100) |
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T-Pro LumiFast Plus Chemiluminescence Detection Kit (ECL Kit) |
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T-Pro LumiFast Plus Chemiluminescence Detection Kit (ECL Kit) |
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HiScript II Q Select RT SuperMix for qPCR (+g DNA wiper) |
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OneScriptPlus cDNA Synthesis SuperMix |
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OneScriptPlus cDNA Synthesis SuperMix |
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Description: Corning and Axygen Liquid Handling Equipment; Axypet Pipettors and Motopet Pipet Controller |
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T-Pro Laemmli (SDS sample) Reagent (non-reducing 4X) |
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Frit Kit |
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Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool. |
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T-Pro Washing buffer in PBS and Tween-20 (10X) |
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Column Packing Kit |
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Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar. |
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T-Pro Phosphate-Buffered Saline (PBS, 10X) for western blot washing |
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PCR SuperMix for PAGE (+dye) |
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Lentivirus qPCR Quantification Kit |
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qPCR Control Template Kit |
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Entrans qPCR Probe Kit |
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QPCR Kit DNA Staphylococ |
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QPCR Kit DNA Clostridium |
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| MOL6826 | Scientific Laboratory Supplies | EACH | EUR 772.92 |
QPCR Kit DNA Clostridium |
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| MOL6830 | Scientific Laboratory Supplies | EACH | EUR 839.04 |
QPCR Kit DNA Clostridium |
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| MOL6838 | Scientific Laboratory Supplies | EACH | EUR 804.84 |
QPCR Kit DNA Cytomegalov |
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| MOL6854 | Scientific Laboratory Supplies | EACH | EUR 839.04 |
QPCR Kit DNA Enterococcu |
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QPCR Kit DNA Haemophilus |
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| MOL6918 | Scientific Laboratory Supplies | EACH | EUR 839.04 |
QPCR Kit RNA Coronavirus |
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| MOL6946 | Scientific Laboratory Supplies | EACH | EUR 820.8 |
QPCR Kit DNA Klebsiella |
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| MOL8156 | Scientific Laboratory Supplies | EACH | EUR 1220.94 |
QPCR Kit DNA Leishmania |
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| MOL8164 | Scientific Laboratory Supplies | EACH | EUR 839.04 |
QPCR Kit DNA Legionella |
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QPCR Kit DNA Legionella |
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| MOL8176 | Scientific Laboratory Supplies | EACH | EUR 839.04 |
QPCR Kit DNA Mycobacteri |
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| MOL8192 | Scientific Laboratory Supplies | EACH | EUR 772.92 |
QPCR Kit DNA Mycoplasma |
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| MOL8204 | Scientific Laboratory Supplies | EACH | EUR 839.04 |
QPCR Kit DNA Mycobacteri |
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| MOL8212 | Scientific Laboratory Supplies | EACH | EUR 839.04 |
QPCR Kit DNA Mycoplasma |
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QPCR Kit DNA Mycobacteri |
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QPCR Kit DNA Methicillin |
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QPCR Kit DNA Methicillin |
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QPCR Kit DNA Methicillin |
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QPCR Kit DNA Methicillin |
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QPCR Kit DNA Oxalobacter |
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QPCR Kit DNA Plasmodium |
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QPCR Kit DNA Pneumocysti |
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QPCR Kit RNA Respiratory |
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QPCR Kit RNA Respiratory |
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QPCR Kit RNA Respiratory |
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| MOL8308 | Scientific Laboratory Supplies | EACH | EUR 1220.94 |
QPCR Kit DNA Staphylococ |
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| MOL8316 | Scientific Laboratory Supplies | EACH | EUR 839.04 |
COVID-19-assoziierte Pneumonie trotz persistierend negativen PCR–Tests aus oropharyngealen Abstrichen.
- A 46-year old construction worker presented at the emergency department with two orthostatic syncopes. The patient complained of prolonged fever and coughs for 7 days which had not improved after oral treatment with sultamicillin for 5 days, prescribed by the patient’s general practitioner. Physical examination showed high blood pressure due to previously known hypertension.
- Other vital signs without pathological findings. Pulmonary auscultation showed basal soft crackling noises of the left lung FINDINGS AND DIAGNOSIS: Laboratory examination showed increased values for LDH, pro-BNP and CRP and normal values for leucocytes and procalcitonin.
- Conventional X-Ray of the chest showed bipulmonal lateral atypical infiltrates. After the first PCR turned in negative another PCR-analysis for SARS-CoV-2 of a deep oral swab-sample was performed since the clinical, laboratory and radiological findings were typical for COVID-19. Again, SARS-CoV-2-RNA was not detected.
- A CT-scan of the chest showed bipulmonal lateral ground-glass attenuation, again typical for COVID-19 associated pneumonia. After a third attempt for a PCR-analysis of a deep oral swab-sample was negative, analysis of a sputum was performed which finally confirmed the diagnosis of COVID-19 associated pneumonia. The patient was admitted for evaluation of syncopes and suspect of COVID-19 associated pneumonia. The patient was prophylactically isolated while the result of SARS-CoV-2-PCR from a deep oral swab was pending.
- Suspecting a possible secondary bacterial infection at the beginning, intravenous antibiotic treatment with ampicillin/sulbactam was initiated. While further examinations showed no indication for bacterial infection, antibiotics were discontinued after 3 days. Due to clinical recovery antiviral therapy was not performed after confirming the diagnosis.
- The patient was discharged 17 days after onset of first symptoms without any requirements for further isolation. This casuistic describes a case of COVID-19 associated pneumonia presenting with typical clinical features, laboratory and radiological findings.
- Detection of viral RNA was not successful from deep oral swab-samples despite repeated attempts. Finally, PCR-analysis of sputum confirmed the diagnosis. Analysis of deeper airway samples (sputum, bronchoalveolar lavage, tracheal secretions) or stool for SARS-CoV-2 should be performed in cases of evident clinical suspicion of COVID-19 and negative PCR results from deep oral swabs
Novel Coronavirus COVID-19 (2019-nCoV) Real Time RT-PCR Kit |
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| RR-0478-02 | Liferiver | 25 tests/kit | EUR 1189.2 |
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Description: Novel Coronavirus (2019-nCoV) Real Time RT-PCR Kit is used for the qualitative detection of a novel coronavirus, which was identified in 2019 at Wuhan City, Hubei Province, China, in upper respiratory tract specimens (nasopharyngeal extracts, deep cough sputum, etc.) and lower respiratory tract specimens (alveoli irrigation fluid, etc.) by real time PCR systems. |
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0,1ML THIN WALL TUBES 8 PER STRIP. CLEAR & REAL TIME STRIP CAPS |
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| PCR-0108-LP-RT-C | CORNING | 125/pk | EUR 850.8 |
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Description: PCR Plates & Tubes; PCR Strip Tubes and Strip Caps - Axygen |
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0,1ML THIN WALL TUBES 8 PER STRIP. WHITE & REAL TIME STRIP CAPS |
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| PCR-0108-LP-RT-W | CORNING | 125/pk | EUR 924 |
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Description: PCR Plates & Tubes; PCR Strip Tubes and Strip Caps - Axygen |
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hsa-mir-521 Real-Time RT-PCR Detection and U6 Calibration Kit |
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| 20-abx097601 | Abbexa |
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TurboQ Real-time PCR Machine with Laptop |
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| MOL2700 | Scientific Laboratory Supplies | EACH | EUR 30805.08 |
lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain |
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| M-PCR-173L | MiTeGen | 960 reactions x 20 µl | EUR 789.36 |
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Description: lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain |
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lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain |
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| M-PCR-173S | MiTeGen | 192 reactions x 20 µl | EUR 197.34 |
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Description: lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain |
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dsGreen for Real-Time PCR, 100×, 2 mL |
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| 61010 | Lumiprobe | 2 mL | EUR 435.6 |
dsGreen for Real-Time PCR, 100×, 1.5 mL |
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| 51010 | Lumiprobe | 1.5 mL | EUR 397.2 |
dsGreen for Real-Time PCR, 100×, 1 mL |
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| 41010 | Lumiprobe | 1 mL | EUR 298.8 |
dsGreen for Real-Time PCR, 100×, 50 mL |
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| 91010 | Lumiprobe | 50 mL | EUR 3802.8 |
dsGreen for Real-Time PCR, 100×, 10 mL |
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| 81010 | Lumiprobe | 10 mL | EUR 1042.8 |
dsGreen for Real-Time PCR, 100×, 5 mL |
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| 71010 | Lumiprobe | 5 mL | EUR 750 |
PMA Real-Time PCR Bacterial Viability Kit - Salmonella enterica (invA) |
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| 31033 | Biotium | 1kit | EUR 513.6 |
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Description: Minimum order quantity: 1 unit of 1kit |
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PMA Real-Time PCR Bacterial Viability Kit - Mycobacterium tuberculosis (groEL2) |
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| 31034 | Biotium | 1kit | EUR 478.8 |
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Description: Minimum order quantity: 1 unit of 1kit |
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PMA Real-Time PCR Bacterial Viability Kit - Staphylococcus aureus (nuc) |
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| 31035 | Biotium | 1kit | EUR 478.8 |
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Description: Minimum order quantity: 1 unit of 1kit |
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PMA Real-Time PCR Bacterial Viability Kit - Staphylococcus aureus (mecA) |
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| 31036 | Biotium | 1kit | EUR 478.8 |
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Description: Minimum order quantity: 1 unit of 1kit |
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PMA Real-Time PCR Bacterial Viability Kit - E. coli (uidA) |
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| 31050 | Biotium | 1kit | EUR 513.6 |
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Description: Minimum order quantity: 1 unit of 1kit |
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PMA Real-Time PCR Bacterial Viability Kit - Listeria monocytogenes (hly) |
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| 31051 | Biotium | 1kit | EUR 478.8 |
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Description: Minimum order quantity: 1 unit of 1kit |
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PMA Real-Time PCR Bacterial Viability Kit - Legionella pneumophila (mip) |
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| 31053 | Biotium | 1kit | EUR 513.6 |
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Description: Minimum order quantity: 1 unit of 1kit |
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hsa-let-7a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097603 | Abbexa |
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hsa-let-7b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097604 | Abbexa |
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hsa-let-7c Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097605 | Abbexa |
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hsa-let-7d Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097606 | Abbexa |
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hsa-let-7e Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097607 | Abbexa |
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hsa-let-7f Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097608 | Abbexa |
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hsa-let-7g Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097609 | Abbexa |
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hsa-let-7i Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097610 | Abbexa |
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hsa-let-7f Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097611 | Abbexa |
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hsa-mir-1 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097612 | Abbexa |
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hsa-mir-7 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097613 | Abbexa |
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hsa-mir-9 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097614 | Abbexa |
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hsa-mir-9* Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097615 | Abbexa |
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hsa-mir-10a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097616 | Abbexa |
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hsa-mir-10b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097617 | Abbexa |
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hsa-mir-10a* Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097618 | Abbexa |
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hsa-mir-15a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097619 | Abbexa |
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hsa-mir-15b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097620 | Abbexa |
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hsa-mir-16 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097622 | Abbexa |
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hsa-mir-18a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097625 | Abbexa |
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hsa-mir-18b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097626 | Abbexa |
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hsa-mir-19a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097627 | Abbexa |
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hsa-mir-19b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097628 | Abbexa |
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hsa-mir-20a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097629 | Abbexa |
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hsa-mir-20b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097630 | Abbexa |
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hsa-mir-21 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097631 | Abbexa |
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hsa-mir-22 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097632 | Abbexa |
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hsa-mir-23a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097633 | Abbexa |
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hsa-mir-23b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097634 | Abbexa |
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hsa-mir-25 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097636 | Abbexa |
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hsa-mir-26a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097637 | Abbexa |
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hsa-mir-26b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097638 | Abbexa |
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hsa-mir-27a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097639 | Abbexa |
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hsa-mir-27b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097640 | Abbexa |
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hsa-mir-29a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097642 | Abbexa |
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hsa-mir-29b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097643 | Abbexa |
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hsa-mir-29c Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097644 | Abbexa |
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hsa-mir-30a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097645 | Abbexa |
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hsa-mir-30b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097647 | Abbexa |
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hsa-mir-30c Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097648 | Abbexa |
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hsa-mir-30d Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097650 | Abbexa |
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hsa-mir-30e Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097651 | Abbexa |
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hsa-mir-31 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097652 | Abbexa |
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hsa-mir-32 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097653 | Abbexa |
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hsa-mir-33a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097654 | Abbexa |
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hsa-mir-34a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097657 | Abbexa |
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hsa-mir-34b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097659 | Abbexa |
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hsa-mir-92a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097661 | Abbexa |
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hsa-mir-92b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097662 | Abbexa |
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hsa-mir-93 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097663 | Abbexa |
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hsa-mir-95 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097664 | Abbexa |
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hsa-mir-96 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097665 | Abbexa |
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hsa-mir-98 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097666 | Abbexa |
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hsa-mir-99a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097667 | Abbexa |
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hsa-mir-99b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097669 | Abbexa |
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hsa-mir-100 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097671 | Abbexa |
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hsa-mir-101 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097673 | Abbexa |
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hsa-mir-103 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097675 | Abbexa |
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hsa-mir-105 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097676 | Abbexa |
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hsa-mir-106a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097677 | Abbexa |
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hsa-mir-106b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097678 | Abbexa |
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hsa-mir-107 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097679 | Abbexa |
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hsa-mir-124 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097680 | Abbexa |
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hsa-mir-125a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097681 | Abbexa |
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