eight patients of COVID-19 with positive RT-PCR test after discharge

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ichs2020

The clinical characteristic of eight patients of COVID-19 with positive RT-PCR test after discharge.

  • Corona virus disease 2019 (COVID-19) was caused by Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2). The phenomenon of positive real time reverse transcription polymerase chain reaction (RT-PCR) result of SARS-CoV-2 in recovered patients had occurred and the research about these patients was rare.
  • In our study, we did a retrospective review of medical records from COVID-19 patients admitted to one ward of Tongji Hospital of Hua Zhong University of Science and Technology from 10 February to 13 April 2020. From 10 February to 13 April 2020, there were 108 patients of COVID-19 admitted in the one ward of Tongji Hospital. Among them, eight cases were readmission patients because the RT-PCR result of SARS-CoV-2 was positive again after discharge. On the second admission, they had no symptoms and their chest CT was almost normal.
  • Data from laboratory tests of the re-admission patients showed that all eight patients had normal white blood cell count, lymphocyte count. The inflammatory factors like procalcitonin and interleukin 6 were normal. After treatment, two patients met the standard and were discharged. The other six patients were still in the hospital because their RT-PCR of SARS-CoV-2 did not get three consecutive negative results and the course of two patients had persisted more than 90 days.
  • We still needed to be alert that these patients could infect other people as a source of infection, and we also needed to be alert that these patients become chronic virus carriers. It also aroused our concern about the discharge standard of COVID-19. This article is protected by copyright. All rights reserved.
ichs2020

ichs2020

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COVID-19-assoziierte Pneumonie trotz persistierend negativen PCR–Tests aus oropharyngealen Abstrichen.

  • A 46-year old construction worker presented at the emergency department with two orthostatic syncopes. The patient complained of prolonged fever and coughs for 7 days which had not improved after oral treatment with sultamicillin for 5 days, prescribed by the patient’s general practitioner. Physical examination showed high blood pressure due to previously known hypertension.
  • Other vital signs without pathological findings. Pulmonary auscultation showed basal soft crackling noises of the left lung FINDINGS AND DIAGNOSIS: Laboratory examination showed increased values for LDH, pro-BNP and CRP and normal values for leucocytes and procalcitonin.
  • Conventional X-Ray of the chest showed bipulmonal lateral atypical infiltrates. After the first PCR turned in negative another PCR-analysis for SARS-CoV-2 of a deep oral swab-sample was performed since the clinical, laboratory and radiological findings were typical for COVID-19. Again, SARS-CoV-2-RNA was not detected.
  • A CT-scan of the chest showed bipulmonal lateral ground-glass attenuation, again typical for COVID-19 associated pneumonia. After a third attempt for a PCR-analysis of a deep oral swab-sample was negative, analysis of a sputum was performed which finally confirmed the diagnosis of COVID-19 associated pneumonia. The patient was admitted for evaluation of syncopes and suspect of COVID-19 associated pneumonia. The patient was prophylactically isolated while the result of SARS-CoV-2-PCR from a deep oral swab was pending.
  • Suspecting a possible secondary bacterial infection at the beginning, intravenous antibiotic treatment with ampicillin/sulbactam was initiated. While further examinations showed no indication for bacterial infection, antibiotics were discontinued after 3 days. Due to clinical recovery antiviral therapy was not performed after confirming the diagnosis.
  • The patient was discharged 17 days after onset of first symptoms without any requirements for further isolation. This casuistic describes a case of COVID-19 associated pneumonia presenting with typical clinical features, laboratory and radiological findings.
  • Detection of viral RNA was not successful from deep oral swab-samples despite repeated attempts. Finally, PCR-analysis of sputum confirmed the diagnosis. Analysis of deeper airway samples (sputum, bronchoalveolar lavage, tracheal secretions) or stool for SARS-CoV-2 should be performed in cases of evident clinical suspicion of COVID-19 and negative PCR results from deep oral swabs

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RR-0479-02 25 tests/kit
EUR 469.56
Description: Novel Coronavirus (2019-nCoV) Real Time Multiplex RT-PCR Kit is used for the qualitative detection of a novel coronavirus, which was identified in 2019 at Wuhan City, Hubei Province, China, in upper respiratory tract specimens (nasopharyngeal extracts, deep cough sputum, etc.) and lower respiratory tract specimens (alveoli irrigation fluid, etc.) by real time PCR systems. It detects N gene, E gene and RdRp gene of 2019-nCoV. RR-0479-02 has been also approverd by CFDA for emergency use and is WHO standard.

TurboQ Real-time PCR Machine with Laptop
MOL2700 EACH
EUR 33836.4

dsGreen for Real-Time PCR, 100×, 2 mL
61010 2 mL
EUR 435.6

dsGreen for Real-Time PCR, 100×, 5 mL
71010 5 mL
EUR 750

dsGreen for Real-Time PCR, 100×, 10 mL
81010 10 mL
EUR 1042.8

dsGreen for Real-Time PCR, 100×, 1 mL
41010 1 mL
EUR 298.8

Azura PureView 1 Kb DNA Ladder - 100 Lanes
AZ-1101 100 Lanes
EUR 140.4

Azura PureView 1 Kb DNA Ladder - 500 Lanes
AZ-1105 500 Lanes
EUR 277.2

Azura PureView 1 Kb DNA Ladder - 1000 Lanes
AZ-1105-2 1000 Lanes
EUR 326.4

Azura PureView 250bp DNA Ladder - 100 Lanes
AZ-1121 100 Lanes
EUR 140.4

Azura PureView 250bp DNA Ladder - 500 Lanes
AZ-1125 500 Lanes
EUR 277.2

Azura PureView 250bp DNA Ladder - 1000 Lanes
AZ-1125-2 1000 Lanes
EUR 326.4

Azura PureView 100bp DNA Ladder - 100 Lanes
AZ-1131 100 Lanes
EUR 140.4

Azura PureView 100bp DNA Ladder - 500 Lanes
AZ-1135 500 Lanes
EUR 277.2

Azura PureView 100bp DNA Ladder - 1000 Lanes
AZ-1135-2 1000 Lanes
EUR 326.4

Azura PureView 50bp DNA Ladder - 100 Lanes
AZ-1151 100 Lanes
EUR 140.4

Azura PureView 50bp DNA Ladder - 500 Lanes
AZ-1155 500 Lanes
EUR 277.2

Azura PureView 50bp DNA Ladder - 1000 Lanes
AZ-1155-2 1000 Lanes
EUR 326.4

AzuraView GreenFast qPCR Blue Mix Lo Rox - 200 Rxn
AZ-2301 200 Rxn
EUR 214.8

AzuraView GreenFast qPCR Blue Mix Lo Rox - 500 Rxn
AZ-2305 500 Rxn
EUR 384

AzuraView GreenFast qPCR Blue Mix Lo Rox - 2000 Rxn
AZ-2320 2000 Rxn
EUR 1195.2

AzuraView GreenFast qPCR Blue Mix Lo Rox - 5000 Rxn
AZ-2325 5000 Rxn
EUR 2817.6

AzuraView GreenFast qPCR Blue Mix Hi Rox - 200 Rxn
AZ-2401 200 Rxn
EUR 214.8

AzuraView GreenFast qPCR Blue Mix Hi Rox - 500 Rxn
AZ-2405 500 Rxn
EUR 384

AzuraView GreenFast qPCR Blue Mix Hi Rox - 2000 Rxn
AZ-2420 2000 Rxn
EUR 1195.2

AzuraView GreenFast qPCR Blue Mix Hi Rox - 5000 Rxn
AZ-2421 5000 Rxn
EUR 2817.6

Mouse Oxidative Stress Primer Library
MOSL-I 1 set
EUR 657.6

Mouse T-Cell Receptor Signaling Primer Library
MTCR-I 1 set
EUR 657.6

Mouse TGF Beta Signaling Primer Library
MTGFB-I 1 set
EUR 657.6

Mouse Tumor Invasion/Metastasis Primer Library
MTIM-I 1 set
EUR 540

Mouse Tumor Immunity Primer Library
MTIMU-I 1 set
EUR 657.6

Mouse p53 Signaling Primer Library
MTPS-I 1 set
EUR 540

Mouse WNT Signaling Primer Library
MWNT-I 1 set
EUR 657.6

Mouse Apoptosis Primer Library
MPA-I 1 set
EUR 540

Mouse Ras Signaling Primer Library
MRAS-I 1 set
EUR 657.6

Mouse Oncogene Primer Library
MONC-I 1 set
EUR 657.6

Mouse Integrin Signaling Primer Library
MINT-I 1 set
EUR 891.6

Mouse Jak/Stat Signaling Primer Library
MJAK-I 1 set
EUR 774

Mouse MAP Kinase Signaling Primer Library
MMAP-I 1 set
EUR 657.6

Mouse Myeloid Derived Suppressor Cell Primer Library
MMDSC-I 1 set
EUR 657.6

Mouse NFKappaB Primer Library
MNFKB-I 1 set
EUR 540

Mouse Notch Signaling Primer Library
MNOT-I 1 set
EUR 657.6

Mouse Neurotransmitter Signaling Primer Library
MNTS-I 1 set
EUR 657.6

Human T-Cell Anergy/Immune Tolerance Primer Library
HTAIT-I 1 set
EUR 657.6

Human T-Cell Receptor Signaling Primer Library
HTCR-I 1 set
EUR 657.6

Human T-Cell Development and Differentiation Primer Library
HTDD-I 1 set
EUR 657.6

Human TGF Beta Signaling Primer Library
HTGFB-I 1 set
EUR 657.6

Human Th1 and Th2 Cell Differentiation Primer Library
HTHD-I 1 set
EUR 657.6

Human Tumor Invasion/Metastasis Primer Library
HTIM-I 1 set
EUR 540

Human Tumor Immunity Primer Library
HTIMU-I 1 set
EUR 657.6

Human Toll-like Receptor Signaling Primer Library
HTLR-I 1 set
EUR 657.6

Human TNF Signaling Primer Library
HTNF-I 1 set
EUR 657.6

Human p53 Signaling Primer Library
HTPS-I 1 set
EUR 540

Human VEGF Signaling Primer Library
HVEGF-I 1 set
EUR 657.6

Human WNT Signaling Primer Library
HWNT-I 1 set
EUR 657.6

Human Cytoskeleton Primer Library
HSCK-I 1 set
EUR 774

Human Stem Cell Primer Library
HSCL-I 1 set
EUR 540

Human SMAD Signaling Primer Library
HSMAD-I 1 set
EUR 657.6

Human Statin Signaling Library
HSTAT-I 1 set
EUR 657.6

Human Signal Transduction Primer Library
HSTL-I 1 set
EUR 540

Human DNA Repair Primer Library I
HDRL-I 1 set
EUR 657.6

Human DNA Repair Primer Library II
HDRL-II 1 set
EUR 657.6

Human Drug Transporter I Primer Library
HDTP-I 1 set
EUR 657.6

Human Drug Detoxication I
HDTX-I 1 set
EUR 657.6

Human Drug Detoxication II
HDTX-II 1 set
EUR 774

Human Actin Cytoskeleton Signaling Primer Library
HACS-I 1 set
EUR 657.6

Human Adipogenesis Primer Library
HADG-I 1 set
EUR 657.6

Human Adaptive & Innate Immune Response Primer Library
HAIIR-I 1 set
EUR 657.6

Human PI3K-AKT Signaling Primer Library
HAKT-I 1 set
EUR 540

Human Amino Acid Metabolism Primer Library
HAMM-I 1 set
EUR 774

Human Androgen Signaling Primer Library
HAND-I 1 set
EUR 657.6

Human Angiogenesis Primer Library
HAPL-I 1 set
EUR 540

Human Antigen Processing and Presentation Primer Library
HAPP-I 1 set
EUR 657.6

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