eight patients of COVID-19 with positive RT-PCR test after discharge

The clinical characteristic of eight patients of COVID-19 with positive RT-PCR test after discharge.

Corona virus disease 2019 (COVID-19) was caused by Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2). The phenomenon of positive real time reverse transcription polymerase chain reaction (RT-PCR) result of SARS-CoV-2 in recovered patients had occurred and the research about these patients was rare.
In our study, we did a retrospective review of medical records from COVID-19 patients admitted to one ward of Tongji Hospital of Hua Zhong University of Science and Technology from 10 February to 13 April 2020. From 10 February to 13 April 2020, there were 108 patients of COVID-19 admitted in the one ward of Tongji Hospital. Among them, eight cases were readmission patients because the RT-PCR result of SARS-CoV-2 was positive again after discharge. On the second admission, they had no symptoms and their chest CT was almost normal.
Data from laboratory tests of the re-admission patients showed that all eight patients had normal white blood cell count, lymphocyte count. The inflammatory factors like procalcitonin and interleukin 6 were normal. After treatment, two patients met the standard and were discharged. The other six patients were still in the hospital because their RT-PCR of SARS-CoV-2 did not get three consecutive negative results and the course of two patients had persisted more than 90 days.
We still needed to be alert that these patients could infect other people as a source of infection, and we also needed to be alert that these patients become chronic virus carriers. It also aroused our concern about the discharge standard of COVID-19. This article is protected by copyright. All rights reserved.

ichs2020

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COVID-19-assoziierte Pneumonie trotz persistierend negativen PCR–Tests aus oropharyngealen Abstrichen.

A 46-year old construction worker presented at the emergency department with two orthostatic syncopes. The patient complained of prolonged fever and coughs for 7 days which had not improved after oral treatment with sultamicillin for 5 days, prescribed by the patient’s general practitioner. Physical examination showed high blood pressure due to previously known hypertension.
Other vital signs without pathological findings. Pulmonary auscultation showed basal soft crackling noises of the left lung FINDINGS AND DIAGNOSIS: Laboratory examination showed increased values for LDH, pro-BNP and CRP and normal values for leucocytes and procalcitonin.
Conventional X-Ray of the chest showed bipulmonal lateral atypical infiltrates. After the first PCR turned in negative another PCR-analysis for SARS-CoV-2 of a deep oral swab-sample was performed since the clinical, laboratory and radiological findings were typical for COVID-19. Again, SARS-CoV-2-RNA was not detected.
A CT-scan of the chest showed bipulmonal lateral ground-glass attenuation, again typical for COVID-19 associated pneumonia. After a third attempt for a PCR-analysis of a deep oral swab-sample was negative, analysis of a sputum was performed which finally confirmed the diagnosis of COVID-19 associated pneumonia. The patient was admitted for evaluation of syncopes and suspect of COVID-19 associated pneumonia. The patient was prophylactically isolated while the result of SARS-CoV-2-PCR from a deep oral swab was pending.
Suspecting a possible secondary bacterial infection at the beginning, intravenous antibiotic treatment with ampicillin/sulbactam was initiated. While further examinations showed no indication for bacterial infection, antibiotics were discontinued after 3 days. Due to clinical recovery antiviral therapy was not performed after confirming the diagnosis.
The patient was discharged 17 days after onset of first symptoms without any requirements for further isolation. This casuistic describes a case of COVID-19 associated pneumonia presenting with typical clinical features, laboratory and radiological findings.
Detection of viral RNA was not successful from deep oral swab-samples despite repeated attempts. Finally, PCR-analysis of sputum confirmed the diagnosis. Analysis of deeper airway samples (sputum, bronchoalveolar lavage, tracheal secretions) or stool for SARS-CoV-2 should be performed in cases of evident clinical suspicion of COVID-19 and negative PCR results from deep oral swabs

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20-abx097659	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns

hsa-mir-92a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097661	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns

hsa-mir-92b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097662	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns

hsa-mir-93 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097663	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns

hsa-mir-95 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097664	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns

hsa-mir-96 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097665	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns

hsa-mir-98 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097666	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns

hsa-mir-99a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097667	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns

hsa-mir-99b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097669	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns

hsa-mir-100 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097671	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns

hsa-mir-101 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097673	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns

hsa-mir-103 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097675	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns

hsa-mir-105 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097676	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns

hsa-mir-106a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097677	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns

hsa-mir-106b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097678	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns

hsa-mir-107 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097679	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns

hsa-mir-124 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097680	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns

hsa-mir-125a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097681	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns

hsa-mir-127 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097686	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns

hsa-mir-129 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097689	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns

hsa-mir-130a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097690	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns

hsa-mir-130b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097691	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns

hsa-mir-132 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097692	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns

hsa-mir-133a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097693	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns

hsa-mir-133b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097694	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns

hsa-mir-134 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097695	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns

hsa-mir-135b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097696	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns

hsa-mir-137 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097699	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns

hsa-mir-138 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097700	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns

hsa-mir-139 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097701	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns

hsa-mir-140 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097702	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns

hsa-mir-141 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097703	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns

hsa-mir-143 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097706	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns

The clinical characteristic of eight patients of COVID-19 with positive RT-PCR test after discharge.

Fast Pro QPCR SuperMix Kit - ROX premixed

Fast Pro QPCR SuperMix Kit - ROX premixed

Green qPCR SuperMix

Probe qPCR SuperMix

Eva QPCR SuperMix Kit

Eva QPCR SuperMix Kit

Power Eva QPCR SuperMix Kit

Power Eva QPCR SuperMix Kit

Green qPCR SuperMix UDG

Top Green qPCR SuperMix

Tip Green qPCR SuperMix

Library Quantification qPCR SuperMix

cDNA Synthesis SuperMix for qPCR

Top Green qPCR SuperMix (+Dye II)

Tip Green qPCR SuperMix (+Dye II)

cDNA Synthesis SuperMix for qPCR (GC-rich)

HiScript II Q RT SuperMix for qPCR

HiScript II Q Select RT SuperMix for qPCR

PLATEMAX ULTRA CLEAR PERMANENT HEAT SEALING FILM FOR QPCR, 100/500

PLATEMAX ULTRA CLEAR PEELABLE HEAT SEALING FILM FOR QPCR. 100/500

HiScript III RT SuperMix for qPCR (+g DNA wiper)

QCell-Pro One-Step qRT-PCR SuperMix Kit

QCell-Pro One-Step qRT-PCR SuperMix Kit

HiScript II Q Select RT SuperMix for qPCR (+g DNA wiper)

T-Pro Plasmid Mini Kit (100)

T-Pro Plasmid Mini Kit (250)

T-Pro Plasmid Midi Kit (20)

T-Pro Plasmid Maxi Kit (10)

T-Pro BCA Protein Assay kit

T-Pro Genomic DNA Midi Kit (20)

T-Pro Genomic DNA Mini Kit (100)

T-Pro Bradford Protein Assay kit(1X)

T-Pro Stacking Buffer

T-Pro MycoClean spray

PCR SuperMix

T-Pro LumiFast Plus Chemiluminescence Detection Kit (ECL Kit)

T-Pro LumiFast Plus Chemiluminescence Detection Kit (ECL Kit)

T-Pro LumiLong Plus Chemiluminescence Detection Kit (ECL Kit)

T-Pro LumiLong Plus Chemiluminescence Detection Kit (ECL Kit)

T-Pro Endotoxin Removal Plasmid Midi kit (25)

T-Pro Endotoxin Removal Plasmid Maxi kit (10)

T-Pro Plant Genomic DNA Midi Kit (20)

T-Pro Plant Genomic DNA Mini Kit (100)

T-Pro Gel/PCR DNA Purification Maxi Kit (10)

T-Pro Gel/PCR DNA Purification Mini Kit (100)

T-Pro Gel/PCR DNA Purification Mini Kit (250)

T-Pro EZ Gel Solution 8%

T-Pro EZ Gel Solution 8%

T-Pro EZ Gel Solution 10%

T-Pro EZ Gel Solution 10%

T-Pro EZ Gel Solution 12%

T-Pro EZ Gel Solution 12%

T-Pro EZ Gel Solution 15%

T-Pro EZ Gel Solution 15%

T-Pro Separating or Resolving Buffer

T-Pro Transfer Blotting buffer (10X)

T-Pro Western Blot Stripping Reagent

T-Pro Aqua EZ Clean (M)

T-Pro Aqua EZ Clean (S)

T-Pro EZ stain Gel solution

T-Pro EZ stain Gel solution

T-Pro P-Fect Transfection Reagent

PCR SuperMix (-dye)

PCR SuperMix (+dye)

cDNA Synthesis SuperMix

Plus PCR SuperMix

Library Amplification SuperMix

OneScriptcDNA Synthesis SuperMix

OneScriptcDNA Synthesis SuperMix

Evo? cDNA Supermix

Evo? cDNA Supermix

Novo? cDNA Supermix

Novo? cDNA Supermix

T-Pro BCA Protein Assay Reagent A

T-Pro BCA Protein Assay Reagent B

T-Pro Fast Blocking Buffer (in PBS)

T-Pro Fast Blocking Buffer (in TBS)

Entrans qPCR Probe Kit

qPCR Control Template Kit