
The clinical characteristic of eight patients of COVID-19 with positive RT-PCR test after discharge.
- Corona virus disease 2019 (COVID-19) was caused by Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2). The phenomenon of positive real time reverse transcription polymerase chain reaction (RT-PCR) result of SARS-CoV-2 in recovered patients had occurred and the research about these patients was rare.
- In our study, we did a retrospective review of medical records from COVID-19 patients admitted to one ward of Tongji Hospital of Hua Zhong University of Science and Technology from 10 February to 13 April 2020. From 10 February to 13 April 2020, there were 108 patients of COVID-19 admitted in the one ward of Tongji Hospital. Among them, eight cases were readmission patients because the RT-PCR result of SARS-CoV-2 was positive again after discharge. On the second admission, they had no symptoms and their chest CT was almost normal.
- Data from laboratory tests of the re-admission patients showed that all eight patients had normal white blood cell count, lymphocyte count. The inflammatory factors like procalcitonin and interleukin 6 were normal. After treatment, two patients met the standard and were discharged. The other six patients were still in the hospital because their RT-PCR of SARS-CoV-2 did not get three consecutive negative results and the course of two patients had persisted more than 90 days.
- We still needed to be alert that these patients could infect other people as a source of infection, and we also needed to be alert that these patients become chronic virus carriers. It also aroused our concern about the discharge standard of COVID-19. This article is protected by copyright. All rights reserved.

ichs2020
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P-1028 | EpiGentek | 200 Reactions | EUR 496.60 |
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K5054200 | Biochain | 200 reactions | EUR 408.00 |
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![]() CMV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
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CASLV100PA-KIT | SBI | 1 Kit | EUR 1132.00 |
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CASLV105PA-KIT | SBI | 1 Kit | EUR 1132.00 |
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![]() MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
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![]() MSCV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
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CASLV125PA-KIT | SBI | 1 Kit | EUR 1132.00 |
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![]() Multiplex gRNA Kit + EF1-T7-hspCas9-H1-gRNA linearized SmartNuclease vector |
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![]() Multiplex gRNA Kit + CAG-T7-hspCas9-H1-gRNA linearized SmartNuclease vector |
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![]() Multiplex gRNA Kit + CMV-T7-hspCas9-H1-gRNA linearized SmartNuclease vector |
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![]() T7 gRNA SmartNuclease Synthesis Kit (includes CAS510A-1 & T7 IVT synthesis reagents) |
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![]() Cas9 Nickase: CMV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
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![]() Cas9 Nickase: MSCV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
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![]() All-in-One cDNA Synthesis SuperMix |
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B24403 | Bimake | 200 ractions | EUR 456.00 |
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![]() All-in-One cDNA Synthesis SuperMix |
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B24408 | Bimake | 1000 ractions | EUR 1453.00 |
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![]() Green Two-Step qRT-PCR SuperMix |
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![]() Multiplex gRNA Kit + Cas9 Nickase: EF1-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector |
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![]() Multiplex gRNA Kit + Cas9 Nickase: CAG-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector |
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CAS770A-KIT | SBI | 10 rxn | EUR 1132.00 |
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![]() Multiplex gRNA Kit + Cas9 Nickase: CMV-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector |
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CAS790A-KIT | SBI | 10 rxn | EUR 1132.00 |
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![]() Cas9 SmartNuclease Extra Ligation Kit [includes 5x ligation buffer (10 ul) and Fast ligase (2.5ul)] |
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CAS9LIG-KIT | SBI | 1 Kit | EUR 153.00 |
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![]() Fmoc-Pro-Pro-Pro-OH |
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B-3690.0050 | Bachem | 50.0mg | EUR 151.00 |
Description: Sum Formula: C30H33N3O6; CAS# [134303-96-1] |
![]() Fmoc-Pro-Pro-Pro-OH |
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B-3690.0250 | Bachem | 250.0mg | EUR 515.00 |
Description: Sum Formula: C30H33N3O6; CAS# [134303-96-1] |
![]() H-Pro-Pro-Pro-OH |
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H-4795.0250 | Bachem | 250.0mg | EUR 683.00 |
Description: Sum Formula: C15H23N3O4; CAS# [19285-44-0] |
![]() H-Pro-Pro-Pro-OH |
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H-4795.1000 | Bachem | 1.0g | EUR 1989.00 |
Description: Sum Formula: C15H23N3O4; CAS# [19285-44-0] |
![]() Fmoc-Pro-Pro-Pro-OH |
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5-06807 | CHI Scientific | 1g | Ask for price |
![]() Fmoc-Pro-Pro-Pro-OH |
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5-06808 | CHI Scientific | 5g | Ask for price |
![]() COVID-19 RT-qPCR Rapid Detection Kit |
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G628 | ABM | NULL | EUR NULL |
![]() One.Step RT-qPCR Probe Kit (2X concentrated) |
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105-500 | GeneOn | 2 x 1,25 ml | EUR 458.00 |
![]() One.Step RT-qPCR Probe Kit (2X concentrated) |
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105-502 | GeneOn | 10 x 1,25 ml | EUR 1719.00 |
![]() piggyBac qPCR copy number kit, 20 reactions |
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PBC100A-1 | SBI | 20 reactions | EUR 331.00 |
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![]() H-Pro-Pro-Pro-Gly-Met-Arg-Pro-Pro-OH |
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H-3464.0001 | Bachem | 1.0mg | EUR 303.00 |
Description: Sum Formula: C38H61N11O9S; CAS# [141312-42-7] net |
![]() H-Pro-Pro-Pro-Gly-Met-Arg-Pro-Pro-OH |
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H-3464.0005 | Bachem | 5.0mg | EUR 1118.00 |
Description: Sum Formula: C38H61N11O9S; CAS# [141312-42-7] net |
![]() PinPoint-FC 293T Platform Kit for Targeted Gene Insertion (includes PIN320A-1, PIN200A-1, PIN510A-1 & PIN600A-1) |
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PIN320A-KIT | SBI | 1 Kit | EUR 4941.00 |
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![]() PinPoint-FC Murine iPSC Platform Kit for Targeted Gene Insertion (includes PIN340iPS-1, PIN200A-1, PIN510A-1 & PIN600A-1) |
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PIN340iPS-KIT | SBI | 1 Kit | EUR 4941.00 |
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![]() LncRNA Profiler Complete qPCR Array Kit (cDNA synthesis kit, qPCR array and SYBR Green reagent) 20 profiles |
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RA910A-1 | SBI | 20 profiles | EUR 1108.00 |
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![]() H-His-Leu-Pro-Pro-Pro-Val-His-Leu-Pro-Pro-Pro-Val-OH |
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H-2256.0001 | Bachem | 1.0mg | EUR 297.00 |
Description: Sum Formula: C64H98N16O13; CAS# [154244-49-2] |
![]() H-His-Leu-Pro-Pro-Pro-Val-His-Leu-Pro-Pro-Pro-Val-OH |
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H-2256.0005 | Bachem | 5.0mg | EUR 1093.00 |
Description: Sum Formula: C64H98N16O13; CAS# [154244-49-2] |
![]() HotTaq Probe qPCR Mix (ROX) |
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BT11001 | Bioatlas | 250rxn | EUR 109.00 |
Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
![]() HotTaq Probe qPCR Mix (Capillary) |
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BT11003 | Bioatlas | 250rxn | EUR 109.00 |
Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
![]() HotTaq Probe qPCR Universal Mix |
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BT11004 | Bioatlas | 250rxn | EUR 123.00 |
Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
![]() HotTaq EvaGreen qPCR Mix (ROX) |
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BT11101 | Bioatlas | 250rxn | EUR 123.00 |
Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
![]() HotTaq EvaGreen qPCR Mix (Capillary) |
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BT11103 | Bioatlas | 250rxn | EUR 123.00 |
Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
COVID-19-assoziierte Pneumonie trotz persistierend negativen PCR–Tests aus oropharyngealen Abstrichen.
- A 46-year old construction worker presented at the emergency department with two orthostatic syncopes. The patient complained of prolonged fever and coughs for 7 days which had not improved after oral treatment with sultamicillin for 5 days, prescribed by the patient’s general practitioner. Physical examination showed high blood pressure due to previously known hypertension.
- Other vital signs without pathological findings. Pulmonary auscultation showed basal soft crackling noises of the left lung FINDINGS AND DIAGNOSIS: Laboratory examination showed increased values for LDH, pro-BNP and CRP and normal values for leucocytes and procalcitonin.
- Conventional X-Ray of the chest showed bipulmonal lateral atypical infiltrates. After the first PCR turned in negative another PCR-analysis for SARS-CoV-2 of a deep oral swab-sample was performed since the clinical, laboratory and radiological findings were typical for COVID-19. Again, SARS-CoV-2-RNA was not detected.
- A CT-scan of the chest showed bipulmonal lateral ground-glass attenuation, again typical for COVID-19 associated pneumonia. After a third attempt for a PCR-analysis of a deep oral swab-sample was negative, analysis of a sputum was performed which finally confirmed the diagnosis of COVID-19 associated pneumonia. The patient was admitted for evaluation of syncopes and suspect of COVID-19 associated pneumonia. The patient was prophylactically isolated while the result of SARS-CoV-2-PCR from a deep oral swab was pending.
- Suspecting a possible secondary bacterial infection at the beginning, intravenous antibiotic treatment with ampicillin/sulbactam was initiated. While further examinations showed no indication for bacterial infection, antibiotics were discontinued after 3 days. Due to clinical recovery antiviral therapy was not performed after confirming the diagnosis.
- The patient was discharged 17 days after onset of first symptoms without any requirements for further isolation. This casuistic describes a case of COVID-19 associated pneumonia presenting with typical clinical features, laboratory and radiological findings.
- Detection of viral RNA was not successful from deep oral swab-samples despite repeated attempts. Finally, PCR-analysis of sputum confirmed the diagnosis. Analysis of deeper airway samples (sputum, bronchoalveolar lavage, tracheal secretions) or stool for SARS-CoV-2 should be performed in cases of evident clinical suspicion of COVID-19 and negative PCR results from deep oral swabs
![]() hsa-mir-521 Real-Time RT-PCR Detection and U6 Calibration Kit |
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20-abx097601 | Abbexa |
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![]() 0,1ML THIN WALL TUBES 8 PER STRIP. CLEAR & REAL TIME STRIP CAPS |
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PCR-0108-LP-RT-C | CORNING | 125/pk | EUR 709.00 |
Description: PCR Plates & Tubes; PCR Strip Tubes and Strip Caps - Axygen |
![]() 0,1ML THIN WALL TUBES 8 PER STRIP. WHITE & REAL TIME STRIP CAPS |
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PCR-0108-LP-RT-W | CORNING | 125/pk | EUR 770.00 |
Description: PCR Plates & Tubes; PCR Strip Tubes and Strip Caps - Axygen |
![]() dsGreen for Real-Time PCR, 100×, 50 mL |
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91010 | lumiprobe | 50 mL | EUR 3169.00 |
![]() dsGreen for Real-Time PCR, 100×, 10 mL |
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81010 | lumiprobe | 10 mL | EUR 869.00 |
![]() dsGreen for Real-Time PCR, 100×, 5 mL |
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71010 | lumiprobe | 5 mL | EUR 625.00 |
![]() dsGreen for Real-Time PCR, 100×, 1.5 mL |
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51010 | lumiprobe | 1.5 mL | EUR 331.00 |
![]() dsGreen for Real-Time PCR, 100×, 1 mL |
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41010 | lumiprobe | 1 mL | EUR 249.00 |
![]() dsGreen for Real-Time PCR, 100×, 100 uL |
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11010 | lumiprobe | 100 uL | EUR 59.00 |
![]() dsGreen for Real-Time PCR, 100×, 2 mL |
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61010 | lumiprobe | 2 mL | EUR 363.00 |
![]() hsa-mir-34a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097657 | Abbexa |
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![]() hsa-mir-34b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097659 | Abbexa |
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![]() hsa-mir-92a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097661 | Abbexa |
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![]() hsa-mir-92b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097662 | Abbexa |
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![]() hsa-mir-93 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097663 | Abbexa |
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![]() hsa-mir-95 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097664 | Abbexa |
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![]() hsa-mir-96 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097665 | Abbexa |
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![]() hsa-mir-98 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097666 | Abbexa |
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![]() hsa-mir-99a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097667 | Abbexa |
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![]() hsa-mir-99b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097669 | Abbexa |
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![]() hsa-mir-100 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097671 | Abbexa |
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![]() hsa-mir-101 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097673 | Abbexa |
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![]() hsa-mir-103 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097675 | Abbexa |
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![]() hsa-mir-105 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097676 | Abbexa |
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![]() hsa-mir-106a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097677 | Abbexa |
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![]() hsa-mir-106b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097678 | Abbexa |
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![]() hsa-mir-107 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097679 | Abbexa |
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![]() hsa-mir-124 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097680 | Abbexa |
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![]() hsa-mir-125a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097681 | Abbexa |
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![]() hsa-mir-127 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097686 | Abbexa |
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![]() hsa-mir-129 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097689 | Abbexa |
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![]() hsa-mir-130a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097690 | Abbexa |
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![]() hsa-mir-130b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097691 | Abbexa |
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![]() hsa-mir-132 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097692 | Abbexa |
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![]() hsa-mir-133a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097693 | Abbexa |
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![]() hsa-mir-133b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097694 | Abbexa |
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![]() hsa-mir-134 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097695 | Abbexa |
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![]() hsa-mir-135b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097696 | Abbexa |
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![]() hsa-mir-137 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097699 | Abbexa |
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![]() hsa-mir-138 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097700 | Abbexa |
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![]() hsa-mir-139 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097701 | Abbexa |
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![]() hsa-mir-140 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097702 | Abbexa |
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![]() hsa-mir-141 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097703 | Abbexa |
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![]() hsa-mir-143 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097706 | Abbexa |
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![]() hsa-mir-145 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097708 | Abbexa |
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![]() hsa-mir-146a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097709 | Abbexa |
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![]() hsa-mir-149 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097711 | Abbexa |
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![]() hsa-mir-150 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097712 | Abbexa |
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![]() hsa-mir-151 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097714 | Abbexa |
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![]() hsa-mir-152 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097715 | Abbexa |
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![]() hsa-mir-153 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097716 | Abbexa |
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![]() hsa-mir-154 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097717 | Abbexa |
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![]() hsa-mir-181a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097719 | Abbexa |
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![]() hsa-mir-181b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097720 | Abbexa |
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![]() hsa-mir-181c Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097721 | Abbexa |
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![]() hsa-mir-181d Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097722 | Abbexa |
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![]() hsa-mir-182 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097723 | Abbexa |
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![]() hsa-mir-183 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097724 | Abbexa |
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![]() hsa-mir-184 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097725 | Abbexa |
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![]() hsa-mir-185 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097726 | Abbexa |
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![]() hsa-mir-190a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097728 | Abbexa |
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![]() hsa-mir-190b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097729 | Abbexa |
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![]() hsa-mir-192 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097731 | Abbexa |
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![]() hsa-mir-193a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097732 | Abbexa |
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![]() hsa-mir-193b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097733 | Abbexa |
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![]() hsa-mir-194 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097734 | Abbexa |
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![]() hsa-mir-195 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097735 | Abbexa |
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![]() hsa-mir-197 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097738 | Abbexa |
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![]() hsa-mir-198 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097739 | Abbexa |
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![]() hsa-mir-199a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097740 | Abbexa |
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![]() hsa-mir-199b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097741 | Abbexa |
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![]() hsa-mir-200a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097742 | Abbexa |
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![]() hsa-mir-200b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097743 | Abbexa |
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![]() hsa-mir-202 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097745 | Abbexa |
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![]() hsa-mir-203 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097746 | Abbexa |
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![]() hsa-mir-204 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097747 | Abbexa |
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![]() hsa-mir-205 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097748 | Abbexa |
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![]() hsa-mir-206 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097749 | Abbexa |
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![]() hsa-mir-208a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097750 | Abbexa |
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![]() hsa-mir-208b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097751 | Abbexa |
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![]() hsa-mir-211 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097753 | Abbexa |
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![]() hsa-mir-212 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097754 | Abbexa |
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![]() hsa-mir-214 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097755 | Abbexa |
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![]() hsa-mir-215 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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20-abx097756 | Abbexa |
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